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Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.

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References

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Acknowledgments

The experiments presented in this protocol chapter were supported by grants from Technical and Research Council of Turkey (#113S819) and Hacettepe University Research Funds (#013D04101005, #THD-2017-13430).

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Authors and Affiliations

  1. Hacettepe University, Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey

    Petek Korkusuz & Nilgün Yersal

  2. Atilim University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Ankara, Turkey

    Sevil Köse

  3. Hacettepe University, Institute of Health Sciences, Department of Stem Cell Sciences, Ankara, Turkey

    Selin Önen

Authors
  1. Petek Korkusuz
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  2. Sevil Köse
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  3. Nilgün Yersal
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  4. Selin Önen
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Corresponding author

Correspondence to Petek Korkusuz .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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Korkusuz, P., Köse, S., Yersal, N., Önen, S. (2018). Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_151

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  • DOI: https://doi.org/10.1007/7651_2018_151

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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