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Filamentous Fungi (Magnaporthe grisea and Fusarium oxysporum)

  • Protocol
Agrobacterium Protocols Volume 2

Part of the book series: Methods in Molecular Biology ((MIMB,volume 344))

Abstract

A better understanding of fungal biology will facilitate judicious use of beneficial fungi and will also advance our efforts to control pathogenic fungi. Molecular studies of fungal biology have been greatly aided by transformation-mediated mutagenesis techniques. Transformation via nonhomologous integration of plasmid DNA bearing a selectable marker (e.g., antibiotic resistance gene) has been widely used for the random insertional mutagenesis of fungi—as an alternative to chemical and radiation mutagens—mainly because the integration of plasmid into the genome provides a convenient tag for subsequent identification and isolation of the mutated gene. Homologous recombination between a target gene on the chromosome and the introduced DNA carrying its mutant allele results in targeted gene knock-out. An important advance in fungal transformation methodology is the development of Agrobacterium tumefaciens-mediated transformation (ATMT) protocols for fungi. ATMT has been successfully applied to a phylogenetically diverse group of fungi and offers a number of advantages over conventional transformation techniques in both the random insertional mutagenesis and targeted gene knock-out. In this chapter, we describe ATMT protocols and vectors for fungal gene manipulation using two plant pathogenic fungi, Magnaporthe grisea and Fusarium oxysporum, as target organisms.

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Author information

Authors and Affiliations

  1. Department of Plant Pathology, The Pennsylvania State University, University Park, PA

    Chang Hyun Khang, Sook-Young Park & Seogchan Kang

  2. School of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, Korea

    Hee-Sool Rho & Yong-Hwan Lee

Authors
  1. Chang Hyun Khang
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  2. Sook-Young Park
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  3. Hee-Sool Rho
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  4. Yong-Hwan Lee
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  5. Seogchan Kang
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Editor information

Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames, Iowa

    Kan Wang

  2. Department of Agronomy, Iowa State Universtiy, Ames, Iowa

    Kan Wang

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© 2006 Humana Press Inc., Totowa, NJ

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Khang, C.H., Park, SY., Rho, HS., Lee, YH., Kang, S. (2006). Filamentous Fungi (Magnaporthe grisea and Fusarium oxysporum). In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:403

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  • DOI: https://doi.org/10.1385/1-59745-131-2:403

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-843-0

  • Online ISBN: 978-1-59745-131-4

  • eBook Packages: Springer Protocols

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