Agrobacterium Protocols Volume 2

  • Kan Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 344)

Table of contents

  1. Front Matter
    Pages i-xxiii
  2. Root Plants

    1. Front Matter
      Pages 1-1
    2. Owen Wally, Jayaraj Jayaraman, Zamir K. Punja
      Pages 3-12
    3. Weston Msikita, Uzoma Ihemere, Dimuth Siritunga, Richard T. Sayre
      Pages 13-24
    4. Steve Millam
      Pages 25-35
    5. Guo-qing Song, Ken-ichi Yamaguchi
      Pages 37-44
  3. Turf Grasses

    1. Front Matter
      Pages 45-45
    2. Yaxin Ge, Zeng-Yu Wang
      Pages 47-53
    3. Fredy Altpeter
      Pages 55-64
    4. Mariya N. Somleva
      Pages 65-74
    5. Yaxin Ge, Zeng-Yu Wang
      Pages 75-81
    6. Chip Longo, Colin Lickwar, Qian Hu, Kimberly Nelson-Vasilchik, David Viola, Joel Hague et al.
      Pages 83-95
  4. Woody Species

    1. Front Matter
      Pages 97-97
    2. Andrew E. Newhouse, Franziska Schrodt, Charles A. Maynard, William A. Powell
      Pages 99-112
    3. Rubén Álvarez, Mariano Toribio, Millán Cortizo, Ricardo-Javier Ordás Fernández
      Pages 113-123
    4. Zenn-Zong Chen, Cheng-Kuen Ho, In-Suk Ahn, Vincent L. Chiang
      Pages 125-134
    5. Jan Grant, Tracy Dale, Pauline Cooper
      Pages 135-141
    6. Richard Meilan, Caiping Ma
      Pages 143-151
    7. Perumal Venkatachalam, Radha Jayashree, Karumamkandathil Rekha, Sreedharannair Sushmakumari, Sankaren Sobha, Parukkuttyamma Kumari Jayasree et al.
      Pages 153-164
  5. Tropic Plants

    1. Front Matter
      Pages 165-165

About this book

Introduction

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Keywords

DNA Flora algae arabidopsis thaliana fungi genetic engineering molecular biology saccharomyces cerevisiae

Editors and affiliations

  • Kan Wang
    • 1
    • 2
  1. 1.Center for Plant Transformation, Plant Science InstituteIowa State UniverstiyAmes
  2. 2.Department of AgronomyIowa State UniverstiyAmes

Bibliographic information

  • DOI https://doi.org/10.1385/1597451312
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-843-0
  • Online ISBN 978-1-59745-131-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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