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Sweet Potato [Ipomoea batatas (L.) Lam.]

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Agrobacterium Protocols Volume 2

Part of the book series: Methods in Molecular Biology ((MIMB,volume 344))

Abstract

Among the available transformation methods reported on sweet potato, Agrobacterium tumefaciens-mediated transformation is more successful and desirable. Stem explants have shown to be ideal for the transformation of sweet potato because of their ready availability as explants, the simple transformation process, and high-frequency-regeneration via somatic embryogenesis. Under the two-step kanamycin-hygromycin selection method and using the appropriate explants type (stem explants), the efficiency of transformation can be considerably improved in cv. Beniazuma. The high efficiency in the transformation of stem explants suggests that the transformation protocol described in this chapter warrants testing for routine stable transformation of diverse varieties of sweet potato.

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References

  1. Sihachakr, D. and Ducreux, G. (1987) Plant regeneration from protoplast culture of sweet potato (Ipomoea batatas (L.) Lam.). Plant Cell Rep. 6, 326–328.

    Article  Google Scholar 

  2. Prakash, C.S. and Varadarajan, U. (1992) Genetic transformation of sweet potato by particle bombardment. Plant Cell Rep. 11, 53–57.

    Article  Google Scholar 

  3. Dhir, S.K., Oglesby, J., and Bhagsari, A.S. (1998) Plant regeneration via somatic embryogenesis, and transient gene expression in sweet potato protoplasts. Plant Cell Rep. 17, 665–669.

    Article  CAS  Google Scholar 

  4. Lawton, R., Winfield, S., Daniell, H., Bhagsari, A.S., and Dhir, S.K. (2000) Expression of green-fluorescent protein gene in sweet potato tissues. Plant Mol. Biol. Rep. 18, 139a–139i.

    Article  Google Scholar 

  5. Otani, M., Mii, M., Handa, T., Kamada, H., and Shimada, T. (1993) Transformation of sweet potato (Ipomoea batatas (L.) Lam.) plants by Agrobacterium rhizogenes. Plant Sci. 94, 151–159.

    Article  CAS  Google Scholar 

  6. Newell, C.A., Lowe, J.M., Merryweather, A., Booke, L.M., and Hamilton, W.D.O. (1995) Transformation of sweet potato (Ipomoea batatas (L.) Lam.) with Agrobacterium tumefaciens and regeneration of plants expressing cowpea trypsin inhibitor and snowdrop lectin. Plant Sci. 107, 215–227.

    Article  CAS  Google Scholar 

  7. Morán, R., García, R., López, A., et al. (1998) Transgenic sweet potato plants carrying the delta-endotoxin gene from Bacillus thuringiensis var. tenebrionis. Plant Sci. 139, 175–184.

    Article  Google Scholar 

  8. Gama, M.I.C., Leite, J.R.P., Cordeiro, A.R., and Cantliffe, D.J. (1996) Transgenic sweet potato plants obtained by Agrobacterium tumefaciens-mediated transformation. Plant Cell Tiss. Org. Cult. 46, 237–244.

    Article  CAS  Google Scholar 

  9. Otani, M., Shimada, T., Kimura, T., and Saito, A. (1998) Transgenic plant production from embryogenic callus of sweet potato (Ipomoea batatas (L.) Lam.) using Agrobacterium tumefaciens. Plant Biotechnol. 15, 11–16.

    CAS  Google Scholar 

  10. Kimura, T., Otani, M., Noda, T., Ideta, O., Shimada, T., and Saito, A. (2001) Absence of amylose in sweet potato [Ipomoea batatas (L.) Lam.] following the introduction of granule-bound starch synthase I cDNA. Plant Cell Rep. 20, 663–666.

    CAS  Google Scholar 

  11. Otani, M., Wakita Y., and Shimada, T. (2003) Production of herbicide-resistant sweet potato (Ipomoea Batatas (L.) Lam.) plants by Agrobacterium tumefaciens-mediated transformation. Breed. Sci. 53, 145–148.

    Article  CAS  Google Scholar 

  12. Song G.-Q., Honda H., and Yamaguchi K. (2004) Efficient Agrobacterium tumefaciens-mediated transformation of sweet potato (Ipomoea Batatas (L.) Lam) from stem explants using a two-step kanamycin-hygromycin selection method. In Vitro Cell. Dev. Biol. Plant 40, 359–365.

    Article  CAS  Google Scholar 

  13. Ohta, S., Mita, S., Hattori, T., and Nakamura, K. (1990) Construction and expression in tobacco of a β-glucuronidase (GUS) reporter gene containing an intron within the coding sequence. Plant Cell Physiol. 31, 805–814.

    CAS  Google Scholar 

  14. Linsmaier, E.M. and Skoog, F. (1965) Organic growth factor requirement of tobacco tissue cultures. Physiol. Plant. 18, 100–127.

    Article  CAS  Google Scholar 

  15. Otani, M. and Shimada, T. (1996) Efficient embryogenic callus formation in sweet potato (Ipomoea batatas (L.) Lam.). Breed. Sci. 46, 257–260.

    Google Scholar 

  16. Hood, E.E., Gelvin, S.B., Melchers, L.S., and Hoekema, A. (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res. 2, 208–218.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Plant Transformation Center, Department of Horticulture, Michigan State University, East Lansing, MI

    Guo-qing Song

  2. Laboratory for Sustainable Horticulture, Minami Kyushu University, Takanabe-cho, Miyazaki, 884-0003, Japan

    Ken-ichi Yamaguchi

Authors
  1. Guo-qing Song
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  2. Ken-ichi Yamaguchi
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Editor information

Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames, Iowa

    Kan Wang

  2. Department of Agronomy, Iowa State Universtiy, Ames, Iowa

    Kan Wang

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© 2006 Humana Press Inc., Totowa, NJ

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Song, Gq., Yamaguchi, Ki. (2006). Sweet Potato [Ipomoea batatas (L.) Lam.]. In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:37

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  • DOI: https://doi.org/10.1385/1-59745-131-2:37

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-843-0

  • Online ISBN: 978-1-59745-131-4

  • eBook Packages: Springer Protocols

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