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American Chestnut [Castanea dentata (Marsh.) Borkh.]

  • Protocol
Agrobacterium Protocols Volume 2

Part of the book series: Methods in Molecular Biology ((MIMB,volume 344))

Abstract

The key to successful transformation of American chestnut is having the correct combination of explant tissue, selectable and scorable markers, and a reliable regeneration system. Rapidly dividing somatic embryos, growing as proembryogenic masses, are a suitable tissue; the bar gene is a suitable selectable marker in conjunction with 1.0 to 10 mg/L phosphirothricin (PPT); and the mgfp5-ER gene is an effective nondestructive scorable marker. We have also found that the more gently the somatic embryos are treated during the inoculation and co-cultivation steps, the higher the transformation efficiency. The average transformation efficiency that can be expected using the described protocol is approx 20 stable and embryogenic transformation events/g of somatic embryo tissue. Cell line and batch-to-batch deviations both upward and downward should be expected. Finally, somatic embryos can be induced to form shoots, which can then be micropropagated and acclimatized.

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References

  1. Griffin, G.J. (1986) Chestnut blight and its control. Hortic. Rev. 8, 291–336.

    Google Scholar 

  2. Murrill, W.A. (1906) A serious chestnut disease. J. N.Y. Bot. Gard. 7, 143–153.

    Google Scholar 

  3. Diller, J.D. and Clapper, R.B. (1965) A progress report on attempts to bring back the chestnut tree in the eastern United States, 1954–1964. J. For. 63, 186–188.

    Google Scholar 

  4. Gonzales, M.L., Vieitez, A.M., and Vieitez, E. (1985) Somatic embryogenesis from chestnut cotyledon tissue cultured in vitro. Sci. Hortic. 27, 97–103.

    Article  Google Scholar 

  5. Piagnani, C. and Eccher, T. (1990) Somatic embryogenesis in chestnut. Acta Hortic. 280, 159–161.

    Google Scholar 

  6. Vieitez, F.J., San-Jose, M.C., Ballester, A., and Vieitez, A.M. (1990) Somatic embryogenesis in cultured immature zygotic embryos in chestnut. J. Plant Physiol. 136, 253–256.

    Google Scholar 

  7. Merkle, S.A., Wiecko, A.T., and Watson-Pauley, B.A. (1991) Somatic embryogenesis in American chestnut. Can. J. For. Res. 21, 1698–1701.

    Article  Google Scholar 

  8. Maynard, C.A. (1991) Using PCR to confirm Agrobacterium transformation of American chestnut (Castanea dentata). Abstract No. 927, In: Program and Abstracts of the Third International Congress of Plant Molecular Biology: Molecular Biology of Plant Growth and Development, Tucson, Arizona, October 6–11, 1991.

    Google Scholar 

  9. Merkle, S., Carraway, D.T., Watson-Pauley, B.A., and Wilde, H.D. (1992) Somatic embryogenesis and gene transfer in American chestnut. In: Proceedings of the International Chestnut Conference, West Virginia University Press, Morgantown, WV. July 10–14, 1992.

    Google Scholar 

  10. Carraway, D.T., Wilde, H.D., and Merkle, S.A. (1994) Somatic embryogenesis and gene transfer in American chestnut. J. Am. Chest. Found. 8, 29–33.

    Google Scholar 

  11. Vieitez, A.M., Ballester, A., Vieitez, M.L., and Vieitez, E. (1983) In vitro plantlet regeneration of mature chestnut. J. Hortic. Sci. 58, 457–463.

    Google Scholar 

  12. Vieitez, A.M. and Vieitez, M.L. (1983) Castanea sativa plantlets proliferated from axillary buds cultivated in vitro. Sci. Hortic. 18, 343–351.

    Article  Google Scholar 

  13. Chauvin, J.E. and Salesses, G. (1988) Advances in chestnut micropropagation (Castanea sp.). Acta. Hortic. 227, 340–345.

    Google Scholar 

  14. Chevre, A.-M. and Salesses, G. (1987) Choice of explants for chestnut micropropagation. Acta. Hortic. 212, 517–523.

    Google Scholar 

  15. Mullins, K.V. (1987) Micropropagation of chestnut (Castanea sativa Mill.). Acta. Hortic. 212, 525–530.

    Google Scholar 

  16. Piagnani, C. and Eccher, T. (1988) Factors affecting the proliferation and rooting of chestnut in vitro. Acta Hortic. 227, 384–386.

    Google Scholar 

  17. Strullu, D.G., Grellier, B., Marciniak, D., and Letouze, R. (1986) Micropropagation of chestnut and conditions of mycorrhizal synthesis in vitro. New Phytol. 102, 95–101.

    Article  Google Scholar 

  18. Vieitez, A.M., Ballester, A., and Vieitez, E. (1987) Vitrification in chestnut shoots regenerated in vitro. Acta. Hortic. 212, 231–234.

    Google Scholar 

  19. Maynard, C., Satchwell, M., and Rieckermann, H. (1993) Micropropagation of American chestnut (Castanea dentata (Marsh.) Borkh.): rooting and acclimatization. In: Proceedings of the Second Northern Forest Genetics Association Conference. St. Paul, Minnesota., July 29–30, 1993, pp. 161–170.

    Google Scholar 

  20. Carraway, D.T. and Merkle, S.A. (1997) Plantlet regeneration from somatic embryos of American chestnut. Can. J. For. Res. 27, 1805–1812.

    Article  Google Scholar 

  21. Robichaud, R.L., Lessard, V.C., and Merkle, S.A. (2004) Treatments affecting maturation and germination of American chestnut somatic embryos. J. Plant Physiol. 161, 957–969.

    Article  PubMed  CAS  Google Scholar 

  22. Seabra, R. C. and Pais, M. S. (1998) Genetic transformation of European chestnut. Plant Cell Rep. 17, 177–182.

    Article  CAS  Google Scholar 

  23. Corredoira, E., Montenegro, D., San-Jose, M.C., Vieitez, A.M., and Ballester, A. (2004). Agrobacterium-mediated transformation of European chestnut embryogenic cultures. Plant Cell Rep. 23, 311–318.

    Article  PubMed  CAS  Google Scholar 

  24. Cheng, M., Hu, T., Layton, J., Liu, C., and Fry, J.E. (2003) Desiccation of plant tissues post-Agrobacterium infection enhances T-DNA delivery and increases stable transformation efficiency in wheat. In Vitro Cell. Dev. Bio. Plant. 39, 595–604.

    Article  CAS  Google Scholar 

  25. Hood, E.E., Gelvin, S.B., Melchers, L.S., and Hoekema, A. (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res. 2, 208–218.

    Article  CAS  Google Scholar 

  26. Polin, L.D., Liang, H., Rothrock, R., et al. (2006) Agrobacterium-mediated transformation of American Chestnut] [Castanea dentata (Marsh.) Barkh.] somatic embryos. Plant Cell Tiss. Org. Cul. 84, 69–78.

    Article  CAS  Google Scholar 

  27. White, J., Chang, S.Y., and Bibb, M.J. (1990) A cassette containing the bar gene of Streptomyces hygroscopicus: A selectable marker for plant transformation. Nucl. Acids Res. 18, 1062.

    Article  PubMed  CAS  Google Scholar 

  28. Garbino, J.E. and Belknap, W.R. (1994) Isolation of a ubiquitin-ribosomal protein gene (ubi3) from potato and expression of its promoter in transgenic plants. Plant Mol. Biol. 24, 119–127.

    Article  Google Scholar 

  29. Haseloff, J. and Siemering, K.R. (1998) The uses of GFP in plants. In: Green Fluorescent Protein: Properties, Applications, and Protocols (Chalfie, M. and Kain, S. eds.), Wiley-Liss, New York, (pp. 191–220).

    Google Scholar 

  30. Dratewka-Kos, E., Rahman, S., Grzekzak, Z.F., Kennedy, T.D., Murray, R.K., and Lane, G. (1989) The polypeptide structure of germin as deduced from cDNA sequencing. J. Biol. Chem. 264, 4896–4900.

    PubMed  CAS  Google Scholar 

  31. Mason, H.S., DeWald, D.B., and Mullet, J.E. (1993) Identification of a methyl jas-monate-responsive domain in the soybean vspB promoter. Plant Cell 5, 241–251.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. SUNY College of Environmental Science and Forestry, State University of New York, Syracuse, NY

    Charles A. Maynard & William A. Powell

  2. SUNY College of Environmental Science and Forestry, Faculty of Forest and Natural Resources Management, Syracuse, NY

    Linda D. Polin, Sharon L. LaPierre & Ronald E. Rothrock

Authors
  1. Charles A. Maynard
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  2. Linda D. Polin
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  3. Sharon L. LaPierre
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  4. Ronald E. Rothrock
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  5. William A. Powell
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Editor information

Editors and Affiliations

  1. Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames, Iowa

    Kan Wang

  2. Department of Agronomy, Iowa State Universtiy, Ames, Iowa

    Kan Wang

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© 2006 Humana Press Inc., Totowa, NJ

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Maynard, C.A., Polin, L.D., LaPierre, S.L., Rothrock, R.E., Powell, W.A. (2006). American Chestnut [Castanea dentata (Marsh.) Borkh.]. In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:239

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  • DOI: https://doi.org/10.1385/1-59745-131-2:239

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-843-0

  • Online ISBN: 978-1-59745-131-4

  • eBook Packages: Springer Protocols

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