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Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine

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DNA-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 148))

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Abstract

In biological systems, photoreactive derivatives have been widely applied to study specific interactions of receptor molecules with their ligands by photoaffinity labeling (1–3). While the receptors are generally proteins (e.g., enzymes, immunoglobulins, or hormone receptors), the ligands differ widely in their molecular structure (e.g., sugars, amino acids, nucleotides, or oligomers of these compounds).

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Author information

Authors and Affiliations

  1. Ministerium für Umwelt und Forsten des Landes Rheinland- Pfalz, Mainz, Germany

    Rainer Meffert

  2. Institut für Biochemie, Johannes Gutenberg-Universität, Mainz, Germany

    Klaus Dose & Hans-Jochen Schäfer

  3. Merck KGaA, Darmstadt, Germany

    Gabriele Rathgeber

Authors
  1. Rainer Meffert
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  2. Klaus Dose
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  3. Gabriele Rathgeber
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  4. Hans-Jochen Schäfer
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Editor information

Editors and Affiliations

  1. Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, QC, Canada

    Tom Moss

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© 2001 Humana Press Inc., Totowa, NJ

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Meffert, R., Dose, K., Rathgeber, G., Schäfer, HJ. (2001). Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine. In: Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 148. Humana Press. https://doi.org/10.1385/1-59259-208-2:323

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  • DOI: https://doi.org/10.1385/1-59259-208-2:323

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-625-3

  • Online ISBN: 978-1-59259-208-1

  • eBook Packages: Springer Protocols

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