Abstract
The polymerase chain reaction (PCR) is now commonly used in laboratories involved in research studies and clinical diagnostic work (1,2). A major advantage of PCR combined with reverse transcription (RT-PCR) is that it can be used to amplify and detect rare mRNA within a specimen. However, conventional RT-PCR cannot be used either to quantitate the frequency of cells expressing a particular mRNA or determine the cellular origin of the amplified signal. Both of these factors may be relevant in the interpretation of gene expression.
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© 1997 Humana Press Inc, Totowa, NJ
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Chen, R.H., Fuggle, S.V. (1997). In Situ PCR Amplification of Intracellular mRNA. In: Gosden, J.R. (eds) PRINS and In Situ PCR Protocols. Methods in Molecular Biology™, vol 71. Humana Press. https://doi.org/10.1385/0-89603-395-3:123
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DOI: https://doi.org/10.1385/0-89603-395-3:123
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