Advertisement

Table of contents

  1. Front Matter
    Pages i-xiii
  2. John R. Gosden, Diane Lawson
    Pages 1-6
  3. Jean-Paul Charlieu, Franck Pellestor
    Pages 7-12
  4. Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, Anton H. N. Hopman
    Pages 13-22
  5. Franck Pellestor, Jean-Paul Charlieu
    Pages 23-29
  6. Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, Anton H. N. Hopman
    Pages 31-37
  7. Yoshiro Shibasaki, John R. Gosden
    Pages 45-51
  8. Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, Anton H. N. Hopman
    Pages 53-59
  9. Deryl L. Troyer, Hongyan Xie, Jie Hu
    Pages 71-76
  10. Christiane Mougin, Jean Marc Didier, Dominique Bettinger, Laurence Madoz, Sylviane Coumes-Marquet, Michel Lab
    Pages 77-97
  11. Fred J. Hendler, Alice Shum-Siu, Briana W. Heniford, Mohammed El Mekki Ahmed, V. J. Patel
    Pages 99-111
  12. David E. Gibellini, Maria Carla Re, Giuliano Furlini, Michele La Placa
    Pages 113-122
  13. Raymond H. Chen, Susan V. Fuggle
    Pages 123-132
  14. Gregory J. Tsongalis
    Pages 133-139
  15. Aldan A. Long, Paul Komminoth
    Pages 141-161
  16. Back Matter
    Pages 163-165

About this book

Introduction

Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.

Keywords

DNA Nucleic acid sequence PCR chemistry chromosome flow cytometry hybridization nucleic acid research tissue

Editors and affiliations

  • John R. Gosden
    • 1
  1. 1.Western General HospitalEdinburghUK

Bibliographic information

  • DOI https://doi.org/10.1385/0896033953
  • Copyright Information Humana Press 1997
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-395-5
  • Online ISBN 978-1-59259-557-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Pharma
Biotechnology
Consumer Packaged Goods