Abstract
Abnormal proliferation of human vascular smooth muscle cells (hVSMCs) is a central event in the development of atherosclerosis (1–3) As a result, there is considerable interest in the establishment of hVSMC cultures as a mode1 of this disease process However, it has been noted in the past (4,5) that hVSMCs, especially when cultured by the enzyme-dispersal technique (hVSMC,ED), grow poorly in culture compared to VSMCs from other species (e.g., rat) This has limited their use for cell-culture studies. We have recently reported that the reduced proliferative capacity of hVSMCED from adult aorta can be attributed to the endogenous production of active TGF-β(6,7). We (6–8) and others (9–11) have shown that TGF-β; is a potent inhibtior of smooth muscle cell proliferation. Moreover, recent studies in animal models of atherosclerosis (12) have suggested that TGF-P plays a pivotal role in regulation of vessel wall architecture (13). We have also shown that hVSMCs derived by the alternative method of explanting (hVSMCEX) have a greater proliferative capacity than the hVSMCED (14) In accordance with our hypotheses, the cells grown from explanted tissue did not produce TGF-β; (14).
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References
Ross, R. (1986) The pathogenesis of atherosclerosis an update. N Engl J Med 314, 488–500
Ross, R. and Glomset, J. A (1976) The pathogenesis of atherosclerosis I. N. Engl J Med 295, 369–377
Ross, R (1993) The pathogenesis of atherosclerosis. a perspective for the 1990s Nature 362, 80l–809
Dartsch, P, Voisard, R, Baunedel, G, Hofling, B, and Betz, E (1990) Growth characteristics and cytoskeletal organisation of cultured smooth muscle cells from human primary stenosing and restenosing lesions Arteriosclerosis 10, 62–75.
Dartsch, P, Weiss, H.-D, and Betz, E. (1990) Human vascular smooth muscle cells in culture growth characteristics and protein pattern by use of serum-free media supplements. Eur J Cell Biol 51, 285–294.
Kirschenlohr, H. L, Metcalfe, J. C., Weissberg, P L, and Grainger, D. J (1993) Adult human aortlc smooth muscle cells in culture produce active TGF-�BD;. Am J Physiol 265, C57l–C576
Grainger, D J, Kirschenlohr, H L, Metcalfe, J C, Weissberg, P L., Wade, D P. and Lawn, R M (1993) Proliferation of human smooth muscle cells promoted by lipoprotein(a) Science 260, 1655–1658
Grainger, D J, Kemp, P. R., Witchell, C M, Weissberg, P L, and Metcalfe, J C (1994) Transforming growth factor �DF; decreases the rate of proliferation of rat vascular smooth muscle cells by extending the G, phase of the cell cycle and delays the rise in cychc AMP before entry into M phase Biochem J 299, 227–235
Assoslan, R K and Sporn, M B (1986) Type p transforming growth factor in human platelets release during platelet degranulation and action on vascular smooth muscle cells J Cell Biol 102, 1217–1223
Bjorkerud, S (1991) Effects of transforming growth factor �DF;1 on human arterial smooth muscle cells in vitro Arteriosclerosis Thromb 11, 892–902
Owens, G K, Geisterfer, A A, Yang, Y W, and Kamonya, A (1988) Transforming growth factor �DF;-induced growth inhibition and cellular hypertrophy in cultured vascular smooth muscle cells J Cell Biol 107, 77l–780
Grainger, D J, Kemp, P R, Lin, A C, Lawn, R M, and Metcalfe, J C (1994) Activation of transforming growth factor �DF; is inhibited in transgenic apolipo protein(a) mice Nature 370, 460–462
Grainger, D J and Metcalfe, J C (1995) A pivotal role for TGF-�DF; in athero genesis? Biol Rev Camb Phdos Soc, in press
Kirschenlohr, H L, Metcalfe, J C, Weissberg, P L, and Grainger, D J (1995) Proliferation of human aortlc VSMCs in culture is modulated by active TGF-P Cardiovasc Res 29, 848–855
Majack, R A (1987) Beta-type transforming growth factor specifies organizational behaviour in vascular smooth muscle cell cultures J Cell Biol 105, 465–471
Lemire, J M, Covin, C W, White, S, Giachelli, C M, and Schwartz, S M (1994) Characterization of cloned aortic smooth muscle cells from young rats Am J Pathol 144, 1068–1081.
Jonasson, L, Holm, J, Skalli, O, Gabbiani, G, and Hansson, G K (1985) Expression of class II transplantation antigen on vascular smooth muscle cells in human atherosclerosis J Clan Invest 76, 125–131
Grainger, D J, Witchell, C M., Weissberg, P L., and Metcalfe, J C (1992) Heterogeneity, dedifferentiation and proliferation of primary rat vascular smooth muscle cells and clones J Mol Cell Cardiol 24(suppl. I), 236
Lyons, R. M, Gentry, L E, Purchino, A F, and Moses, H L (1990) Mechanism of activation of latent recombinant transforming growth factor �DF;1 by plasmin J Cell Biol 110, 1361–1367
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© 1996 Humana Press Inc., Totowa, NJ
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Kirschenlohr, H.L., Metcalfe, J.C., Grainger, D.J. (1996). Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta. In: Jones, G.E. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine, vol 2. Humana Press. https://doi.org/10.1385/0-89603-335-X:319
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DOI: https://doi.org/10.1385/0-89603-335-X:319
Publisher Name: Humana Press
Print ISBN: 978-0-89603-335-1
Online ISBN: 978-1-59259-586-0
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