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Evaluation of genetic diversity in Amygdalus mira (Koehne) Ricker using SSR and ISSR markers

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Abstract

Amygdalus mira (Koehne) Ricker, originated in China, is a wild fruit tree distributed in Tibet with high economic and ecological value. To conserve its future breeding and germplasm resources, much information about genetic diversity of A. mira should be obtained. In this study, two DNA-based molecular markers, simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR), were used for genetic diversity evaluations of 46 samples from four regions including Bomi (BM), Langkazi (LKZ), Mainling (ML) and Linzhi (LZ) in Tibet. Through combined analysis of SSR and ISSR, high levels of polymorphism (75.42 %) were observed, indicating that SSR and ISSR were efficient methods to detect genetic diversity of natural A. mira populations; all the samples were divided into two major clusters at the similarity coefficient of 0.54; the highest H and I values were observed in LZ population; most genetic variations occurred within populations; there is no significant relationship between genetic distance of populations and geographic distribution. For conversation of A. mira, ex situ conversation is a desired strategy needed to be established in the future.

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Abbreviations

SSR:

Simple sequence repeat

ISSR:

Inter simple sequence repeat

RAPD:

Random amplified polymorphic DNA

AFLP:

Amplified fragment length polymorphism

CTAB:

Hexadecyl trimethyl ammonium bromide

EDTA:

Ethylenediamine tetraacetic acid

PCR:

Polymerase chain reaction

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Acknowledgments

This study was supported by the Fundamental Research Funds for the Central Universities (No. DL13EA08-02), the National Natural Science Foundation of China (31170568; 31201594).

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Authors and Affiliations

  1. College of Life Science, Northeast Forestry University, Harbin, 150040, China

    Chun Xing, Yan Tian & Fanjuan Meng

  2. Department of Horticulture, Agricultural and Animal Husbandry Colleage of Tibet University, Linzhi, 860000, China

    Fachun Guan

Authors
  1. Chun Xing
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  2. Yan Tian
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  3. Fachun Guan
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  4. Fanjuan Meng
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Correspondence to Fanjuan Meng.

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Handling editor: Frank H. Helwig.

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Xing, C., Tian, Y., Guan, F. et al. Evaluation of genetic diversity in Amygdalus mira (Koehne) Ricker using SSR and ISSR markers. Plant Syst Evol 301, 1055–1064 (2015). https://doi.org/10.1007/s00606-014-1136-3

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  • DOI: https://doi.org/10.1007/s00606-014-1136-3

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