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Isolation of Porcine Pancreatic Islets for Xenotransplantation

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Xenotransplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 885))

Abstract

This chapter deals with a technique for isolating intact islets of Langerhans from the pig pancreas based on our experience performing approximately 750 isolations. The procedure we describe involves identification of an optimal donor pancreas, purification and in vitro culture of islets, diabetes induction in recipients, and transplantation of islets and their immunomodulation. Besides the sophistication of the technical equipment employed, the major factors influencing the isolation outcome are the pig breed, the number and morphology of the islets in the donor pancreas, the quality of the collagenase/neutral protease, and the skill of the team members.

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Acknowledgments

The authors want to thank Mr. Alois Reichert, Mr. Peter Heuler, and Mr. Jens Weinberger for their excellent technical assistance during organ procurement, and Mrs. Lorraine Stevenson-Knebel for critically reading the manuscript and her untiring assistance with the paperwork. Furthermore, the authors want to thank Professor Dr. Dr. h.c. Arnulf Thiede, former director of the Surgical Clinic I, for his never-ending theoretical and practical support of our work.

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Authors and Affiliations

  1. Experimental Transplantation Immunology and Experimental Surgery, Clinic of General, Visceral, Vascular, and Pediatric Surgery, University of Wuerzburg Hospital, Würzburg, Germany

    Karin Ulrichs, Sibylle Eber, Bianca Schneiker, Sabine Gahn & Irina Chodnevskaja

  2. Clinic of General, Visceral, Vascular and Pediatric(Surgical Clinic I), University of Wuerzburg Hospital, Würzburg, Germany

    Armin Strauß & Vasiliy Moskalenko

Authors
  1. Karin Ulrichs
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  2. Sibylle Eber
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  3. Bianca Schneiker
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  4. Sabine Gahn
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  5. Armin Strauß
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  6. Vasiliy Moskalenko
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  7. Irina Chodnevskaja
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Corresponding author

Correspondence to Karin Ulrichs .

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Editors and Affiliations

  1. Bellvitge (IDIBELL), Hospital Duran i Reynals, Institut d'Investigació Biomèdica de, Gran Via de L’Hospitalet 199, L’Hospitalet de Llobregat, 08908, Barcelona, Spain

    Cristina Costa

  2. Servicio de Medicina Intensiva, Hospital Universitario de Bellvitge, Feixa Llarga s/n, L'Hospitalet de Llobregat, 08907, Barcelona, Spain

    Rafael Máñez

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Ulrichs, K. et al. (2012). Isolation of Porcine Pancreatic Islets for Xenotransplantation. In: Costa, C., Máñez, R. (eds) Xenotransplantation. Methods in Molecular Biology, vol 885. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-845-0_13

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  • DOI: https://doi.org/10.1007/978-1-61779-845-0_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-844-3

  • Online ISBN: 978-1-61779-845-0

  • eBook Packages: Springer Protocols

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