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  • © 2012

Xenotransplantation

Methods and Protocols

  • Covers many methods and subjects related to Xenotransplantation
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 885)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Xenotransplantation: An Overview of the Field

    • Veronica Tisato, Emanuele Cozzi
    Pages 1-16
  3. Cloning and Expression Analyses of Pig Genes

    • Mireia Uribe-Herranz, Cristina Costa
    Pages 17-31
  4. Basic Analyses of Proteins of Interest for Xenotransplantation

    • Mireia Uribe-Herranz, Cristina Costa
    Pages 33-45
  5. Studies on Carbohydrate Xenoantigens

    • Dale Christiansen, Effie Mouhtouris, Paul A. Ramsland, Mauro S. Sandrin
    Pages 47-56
  6. Studies on Coagulation Incompatibilities for Xenotransplantation

    • Cristiana Bulato, Claudia Radu, Paolo Simioni
    Pages 71-89
  7. Cellular Studies for In Vitro Modeling of Xenogeneic Immune Responses

    • Roberta Sommaggio, Magdiel Pérez-Cruz, Cristina Costa
    Pages 91-103
  8. Production of Transgenic and Knockout Pigs by Somatic Cell Nuclear Transfer

    • Angelica M. Giraldo, Suyapa Ball, Kenneth R. Bondioli
    Pages 105-123
  9. Heart Xenotransplantation in Primate Models

    • Johannes Postrach, Andreas Bauer, Michael Schmoeckel, Bruno Reichart, Paolo Brenner
    Pages 155-168
  10. Xenogeneic Lung Transplantation Models

    • Lars Burdorf, Agnes M. Azimzadeh, Richard N. Pierson III
    Pages 169-189
  11. Isolation of Porcine Pancreatic Islets for Xenotransplantation

    • Karin Ulrichs, Sibylle Eber, Bianca Schneiker, Sabine Gahn, Armin Strauß, Vasiliy Moskalenko et al.
    Pages 213-232
  12. Pig Neural Cells Derived from Foetal Mesencephalon as Cell Source for Intracerebral Xenotransplantation

    • Xavier Lévêque, Véronique Nerrière-Daguin, Isabelle Neveu, Philippe Naveilhan
    Pages 233-243
  13. Hepatocyte Xenotransplantation

    • Katia R. F. Lima-Quaresma, Andre Gustavo Bonavita, Matheus Kafuri Cytrangulo, Marcelo Alves Pinto, Luiz Anastácio Alves
    Pages 245-249
  14. In Vitro Repair Model of Focal Articular Cartilage Defects in Humans

    • Prado SM Díaz, Fuentes-Boquete IM, Blanco FJ
    Pages 251-261
  15. Potential Zoonotic Infection of Porcine Endogenous Retrovirus in Xenotransplantation

    • Giada Mattiuzzo, Yasuhiro Takeuchi, Linda Scobie
    Pages 263-279
  16. Some Ethical, Social, and Legal Considerations of Xenotransplantation

    • María Jorqui Azofra, Carlos María Romeo Casabona
    Pages 307-329

About this book

Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last two decades. The field seeks to overcome the limitations and difficulties in organ procurement, which also apply to human cells and tissues, and facilitate the development of new therapies based on cell and engineered-tissue. Xenogeneic cells are simpler than solid organs and seem to pose less hurdles to attain long-term graft survival. In, Xenotransplantation: Methods and Protocols expert researchers study characterizations of xenogeneic interactions at the cellular and molecular levels and describe the use of relevant small-animal and pig-to-primate models. Related ethical and legal considerations are also covered. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Xenotransplantation: Methods and Protocols aids scientists in continuing to study xenotransplantation and its multiple aspects.

Reviews

From the reviews:

“This book gives a comprehensive outline on xenotransplantation, with contributions from a wide range of experts in the field. … The chapters are well-organised and sequenced, and the figures and tables complement the text very well. … it is a well-edited text with a multitude of excellent references. … useful as a reference in a research laboratory, and can be of potential interest to researchers of all grades and levels of expertise in this field.” (Karwan Moutasim, Immunology News, May, 2013)

“This is a methodological book aimed at describing specific topics in xenotransplantation. The purpose is to offer a resource to help those in the field of xenotransplantation or those new researchers interested in starting a career. Given the ongoing need for organs and the advances that could be made with successful xenotransplantation, this is a worthy goal. … this is a thorough book on certain aspects of xenotransplantation, and its methodological approach to certain procedures is helpful to scientists just entering the field.” (Erin C. Maynard, Doody’s Book Reviews, September, 2012)

Editors and Affiliations

  • Bellvitge (IDIBELL), Hospital Duran i Reynals, Institut d'Investigació Biomèdica de, L’Hospitalet de Llobregat, Spain

    Cristina Costa

  • Servicio de Medicina Intensiva, Hospital Universitario de Bellvitge, L'Hospitalet de Llobregat, Spain

    Rafael Máñez

Bibliographic Information

  • Book Title: Xenotransplantation

  • Book Subtitle: Methods and Protocols

  • Editors: Cristina Costa, Rafael Máñez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-845-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-844-3Published: 10 May 2012

  • Softcover ISBN: 978-1-4939-5869-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-845-0Published: 08 May 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 335

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access