Abstract
Calcium transients elicited by IP3 receptors upon electrical stimulation of skeletal muscle cells (slow calcium signals) are often hard to visualize due to their relatively small amplitude compared to the large transient originated from ryanodine receptors associated to excitation-contraction coupling. The study of slow calcium transients, however, is relevant due to their function in regulation of muscle gene expression and in the process of excitation-transcription coupling. Discussed here are the procedures used to record slow calcium signals from both cultured mouse myotubes and from cultured adult skeletal muscle fibers.
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Acknowledgments
This work was supported by FONDECYT grant N° 1080120 and FONDAP grant N° 15010006; CONICYT 24100066 Doctoral Support (F.A.).
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Casas, M., Altamirano, F., Jaimovich, E. (2012). Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle. In: DiMario, J. (eds) Myogenesis. Methods in Molecular Biology, vol 798. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-343-1_22
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DOI: https://doi.org/10.1007/978-1-61779-343-1_22
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