Abstract
Pulsed-Field Gel Electrophoresis (PFGE) subtyping has been used extensively to characterize various bacterial species and to facilitate comparative analysis of geographically diverse populations. To this end, standardized protocols for many different genera and species have been developed, particularly through the PulseNet platform. The Bacillus cereus group of bacteria includes a diverse species set, which are of particular importance in food safety as both human pathogens and spoilage organisms. The application of techniques to differentiate strains of B. cereus can be utilized to assist in both disease outbreak investigations, and also in strategies to monitor and control the organism in food production environments. This chapter describes a PFGE method, which may be applied to differentiate B. cereus strains.
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References
Ceuppens S, Boon N, Uyttendaele M (2013) Diversity of Bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles. FEMS Microbiol Ecol 84:433–450
Tourasse NJ, Helgason E, Økstad OA, Hegna IK, Kolstø A-B (2006) The Bacillus cereus group: novel aspects of population structure and genome dynamics. J Appl Microbiol 101:579–593
International Organization for Standardization (2004) Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 degrees C. ISO 7932:2004, 1–13
Tournier JN, Rossi-Paccani S, Quesnel-Hellmann A, Baldari CT (2009) Anthrax toxins: a weapon to systematically dismantle the host immune defenses. Mol Aspects Med 30:456–466
Arslan S, Eyi A, Küçüksari R (2014) Toxigenic genes, spoilage potential, and antimicrobial resistance of Bacillus cereus group strains from ice cream. Anaerobe 25:42–46
Lynch MJ, Fox EM, O’Connor L, Jordan K, Murphy M (2012) Surveillance of verocytotoxigenic Escherichia coli in Irish bovine dairy herds. Zoonoses Public Health 59:264–271
Fox EM, DeLappe N, Garvey P, Cormican M, Leonard N, Jordan K (2012) PFGE analysis of Listeria monocytogenes isolates of clinical, animal, food and environmental origin from Ireland. J Med Microbiol 61:540–547
Stessl B, Fricker M, Fox E, Karpiskova R, Demnerova K, Jordan K, Ehling-Schulz M, Wagner M (2014) Collaborative survey on the colonization of different types of cheese-processing facilities with Listeria monocytogenes. Foodborne Pathog Dis 11:8–14
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Drean, P., Fox, E.M. (2015). Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains. In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_7
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DOI: https://doi.org/10.1007/978-1-4939-2599-5_7
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2598-8
Online ISBN: 978-1-4939-2599-5
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