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The cell biology of homologous recombination

  • Chapter
Molecular Genetics of Recombination

Part of the book series: Topics in Current Genetics ((TCG,volume 17))

Abstract

Discontinuities in double-stranded DNA, such as DNA double-strand breaks (DSBs), pose a threat to genome stability. Homologous recombination is a process that not only effectively repairs DSBs, but also promotes preservation of genome integrity by repairing DNA discontinuities arising during DNA replication. Genetic analyses identified many genes involved in DSB repair and placed them in different pathways. Biochemical analyses have aided in placing the protein products in a mechanistic framework for the pathways, while molecular biological approaches, such as chromatin immuno-precipitation, have allowed the monitoring of protein composition near DSBs in populations of fixed cells. Progress in cell biological techniques has now made it possible to analyze proteins in their physiological environment of the living cell. Here, we describe how homologous recombination proteins have been characterized using the methods of cell biology. The current challenge is to integrate insights gained on the spatio-temporal behavior of DSB repair proteins using chromatin immuno-precipitation and live cell imaging in the established genetic and biochemical frameworks for mechanisms of DSB repair.

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Authors and Affiliations

  1. Department of Cell Biology and Genetics, Erasmus MC, PO Box 1738, 3000 DR, Rotterdam, The Netherlands

    Sheba Agarwal, Roland Kanaar & Jeroen Essers

  2. Department of Radiation Oncology, Erasmus MC, PO Box 1738, 3000 DR, Rotterdam, The Netherlands

    Roland Kanaar & Jeroen Essers

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  1. Sheba Agarwal
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  2. Roland Kanaar
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  1. Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, CSIC-Universidad de Sevilla, Av. Américo Vespucio s/n, 41092, Sevilla, Spain

    Andrés Aguilera

  2. Department of Genetics & Development, Columbia University Medical Center, 701 West 168th Street, HHSC 1608, New York, NY, 10032-2704, USA

    Rodney Rothstein

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Agarwal, S., Kanaar, R., Essers, J. (2007). The cell biology of homologous recombination. In: Aguilera, A., Rothstein, R. (eds) Molecular Genetics of Recombination. Topics in Current Genetics, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-71021-9_12

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