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Collagen Degradation Assays

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Matrix Metalloproteinase Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 622))

Abstract

The biochemistry of collagen makes the assay of its degradation complex. Hidden epitopes are linear amino acid sequences that are not normally available for antibody binding when they are contained within an intact helical structure, but they become exposed once the collagen triple helix has been cleaved and denatured. This chapter describes the use of an antibody raised against such an epitope, combined with selective proteolytic extraction, to assay collagen degradation.

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Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    Anthony P. Hollander

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  1. Anthony P. Hollander
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Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of East Anglia, Norwich, NR47TJ, United Kingdom

    Ian M. Clark

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Hollander, A.P. (2010). Collagen Degradation Assays. In: Clark, I. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology, vol 622. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-299-5_22

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  • DOI: https://doi.org/10.1007/978-1-60327-299-5_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-298-8

  • Online ISBN: 978-1-60327-299-5

  • eBook Packages: Springer Protocols

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