Matrix Metalloproteinase Protocols

  • Ian M. Clark

Part of the Methods in Molecular Biology book series (MIMB, volume 622)

Table of contents

  1. Front Matter
    Pages i-xii
  2. MMPs and TIMPs: An Overview

    1. Front Matter
      Pages 1-1
    2. Alejandro P. Ugalde, Gonzalo R. Ordóñez, Pedro M. Quirós, Xose S. Puente, Carlos López-Otín
      Pages 3-29
    3. Sean E. Gill, Sean Y. Kassim, Timothy P. Birkland, William C. Parks
      Pages 31-52
  3. Expression and Purification of MMPs and TIMPs

    1. Front Matter
      Pages 53-53
    2. Ursula R. Rodgers, Ian M. Clark
      Pages 55-65
    3. Gavin C. Jones, Mireille N. Vankemmelbeke, David J. Buttle
      Pages 83-98
    4. Jing Nie, Duanqing Pei
      Pages 99-110
    5. Ken-ichi Shimokawa, Hideaki Nagase
      Pages 123-155
  4. Detection of MMPs and TIMPs

    1. Front Matter
      Pages 157-157
    2. Caroline J. Pennington, Dylan R. Edwards
      Pages 159-173
    3. Reinhild Kappelhoff, Ulrich auf dem Keller, Christopher M. Overall
      Pages 175-193
    4. Tiina L. Hurskainen, Suneel S. Apte
      Pages 195-209
    5. Yasunori Okada
      Pages 211-219
  5. Assay of MMP and TIMP Activities

    1. Front Matter
      Pages 231-231
    2. Vera Knäuper, Gillian Murphy
      Pages 233-243
    3. Tim E. Cawston, Rachel L. Lakey, Andrew D. Rowan
      Pages 245-255

About this book

Introduction

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms.  Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field.  Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

Keywords

Escherichia coli Microarray Nucleotide PCR Proteomics Single Nucleotide Polymorphism enzymes hybridization molecular biology

Editors and affiliations

  • Ian M. Clark
    • 1
  1. 1.School of Biological SciencesUniversity of East AngliaNorwichUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-299-5
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60327-298-8
  • Online ISBN 978-1-60327-299-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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