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Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials

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Virus-Derived Nanoparticles for Advanced Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1776))

Abstract

M13 bacteriophages have several qualities that make them attractive candidates as building blocks for tissue regenerating scaffold materials. Through genetic engineering, a high density of functional peptides and proteins can be simultaneously displayed on the M13 bacteriophage’s outer coat proteins. The resulting phage can self-assemble into nanofibrous network structures and can guide the tissue morphogenesis through proliferation, differentiation and apoptosis. In this manuscript, we will describe methods to develop major coat-engineered M13 phages as a basic building block and aligned tissue-like matrices to develop regenerative nanomaterials.

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Acknowledgment

This work was supported under the framework of international cooperation program managed by the National Research Foundation of Korea (NRF-2016K2A9A1A01951919). H.-E.J. was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2016R1C1B1008824). We acknowledge funding support from the Tsinghua-Berkeley Shenzhen Institute.

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Authors and Affiliations

  1. Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA

    Hyo-Eon Jin & Seung-Wuk Lee

  2. Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA, USA

    Hyo-Eon Jin & Seung-Wuk Lee

  3. Tshinghua Berkeley Shenzhen Institute, Berkeley, CA, USA

    Seung-Wuk Lee

  4. College of Pharmacy, Ajou University, Suwon, Korea

    Hyo-Eon Jin

Authors
  1. Hyo-Eon Jin
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  2. Seung-Wuk Lee
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Corresponding author

Correspondence to Hyo-Eon Jin .

Editor information

Editors and Affiliations

  1. Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany

    Christina Wege

  2. Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom

    George P. Lomonossoff

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Jin, HE., Lee, SW. (2018). Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_32

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  • DOI: https://doi.org/10.1007/978-1-4939-7808-3_32

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7806-9

  • Online ISBN: 978-1-4939-7808-3

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