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Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages

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Phage Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1701))

Abstract

The current chapter focuses on the use of filamentous phages to display, modify, and characterize cytokines, which are proteins belonging to a versatile group of essential mediators involved in cell-cell communication. Cytokines exhibit a considerable diversity, both in functions and in structural features underlying their biological effects. A broad variety of cytokines have been successfully displayed on phages, allowing the high-throughput study of their binding properties and biological activities and the discovery of novel therapeutics through directed evolution. The technical singularities and some potential applications of cytokine phage display are illustrated here with the case of Interleukin-2, a prototypic member of the four-alpha-helix bundle cytokine family playing a pivotal role in the immune response and having a long history of therapeutic use.

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Acknowledgment

G. Rojas is partially supported by a Georg Forster research fellowship awarded by the Alexander von Humboldt Foundation, Germany.

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Authors and Affiliations

  1. Center of Molecular Immunology, Calle 216 esq 15, Atabey, Playa, La Habana, CP, 11300, Cuba

    Gertrudis Rojas & Tania Carmenate

Authors
  1. Gertrudis Rojas
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  2. Tania Carmenate
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Corresponding author

Correspondence to Gertrudis Rojas .

Editor information

Editors and Affiliations

  1. Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  2. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia

    Theam Soon Lim

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Rojas, G., Carmenate, T. (2018). Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages. In: Hust, M., Lim, T. (eds) Phage Display. Methods in Molecular Biology, vol 1701. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7447-4_30

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  • DOI: https://doi.org/10.1007/978-1-4939-7447-4_30

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7446-7

  • Online ISBN: 978-1-4939-7447-4

  • eBook Packages: Springer Protocols

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