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Monitoring Phage Biopanning by Next-Generation Sequencing

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Phage Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1701))

Abstract

Phage display has enabled the rapid isolation of antigen-specific antibodies from combinatorial libraries of the variable heavy chain gene (VH) and variable light chain gene (VL). The method is based on genetic engineering of bacteriophages and repeated rounds of antigen-guided selection by phage biopanning.

Next-Generation Sequencing (NGS) coupled with bioinformatics are powerful tools for analyzing the large number of DNA sequences present in an immune library.

Here, we describe a method that demonstrates how NGS analysis enhances phage biopanning of complex antibody libraries as well as facilitates the antibody discovery process.

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Author information

Authors and Affiliations

  1. Department of Molecular Microbiology and Biotechnology, Faculty of Life Science, Tel Aviv University, Tel Aviv, 69978, Israel

    Anna Vaisman-Mentesh & Yariv Wine

Authors
  1. Anna Vaisman-Mentesh
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  2. Yariv Wine
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Corresponding author

Correspondence to Yariv Wine .

Editor information

Editors and Affiliations

  1. Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  2. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia

    Theam Soon Lim

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Vaisman-Mentesh, A., Wine, Y. (2018). Monitoring Phage Biopanning by Next-Generation Sequencing. In: Hust, M., Lim, T. (eds) Phage Display. Methods in Molecular Biology, vol 1701. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7447-4_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7447-4_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7446-7

  • Online ISBN: 978-1-4939-7447-4

  • eBook Packages: Springer Protocols

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