Abstract
Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.
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Acknowledgment
We thank the Kayed Lab members for their contributions to the optimization of the protocols and useful suggestions. This work was supported by the Mitchell Center for Neurodegenerative Diseases.
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Gerson, J.E., Sengupta, U., Kayed, R. (2017). Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6598-4_9
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DOI: https://doi.org/10.1007/978-1-4939-6598-4_9
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