Abstract
Abnormal tau aggregation is a pathological hallmark of neurodegenerative disease classified as tauopathy. Preventing tau aggregation becomes an important therapeutic strategy to cure tau-mediated neurodegeneration. Here, we describe a method to investigate intracellular tau aggregation by using a recently developed tau aggregation cell-based model named tau-BiFC. High-throughput and high-contents screening method for quantifying intracellular tau aggregation would expedite the discovery of drugs that inhibit tau aggregation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Reddy PH (2011) Abnormal tau, mitochondrial dysfunction, impaired axonal transport of mitochondria, and synaptic deprivation in Alzheimer’s disease. Brain Res 1415:136–148. doi:10.1016/j.brainres.2011.07.052
Gendron TF, Petrucelli L (2009) The role of tau in neurodegeneration. Mol Neurodegener 4:13. doi:10.1186/1750-1326-4-13
Mukrasch MD, Biernat J, von Bergen M, Griesinger C, Mandelkow E, Zweckstetter M (2005) Sites of tau important for aggregation populate {beta}-structure and bind to microtubules and polyanions. J Biol Chem 280:24978–24986. doi:10.1074/jbc.M501565200
Johnson GV, Hartigan JA (1999) Tau protein in normal and Alzheimer’s disease brain: an update. J Alzheimers Dis 1:329–351
Buée-Scherrer V, Buée L, Leveugle B, Perl DP, Vermersch P, Hof PR, Delacourte A (1997) Pathological tau proteins in postencephalitic parkinsonism: comparison with Alzheimer’s disease and other neurodegenerative disorders. Ann Neurol 42:356–359. doi:10.1002/ana.410420312
Buée Scherrer V, Hof PR, Buée L, Leveugle B, Vermersch P, Perl DP, Olanow CW, Delacourte A (1996) Hyperphosphorylated tau proteins differentiate corticobasal degeneration and Pick’s disease. Acta Neuropathol 91:351–359
Brandt R, Hundelt M, Shahani N (2005) Tau alteration and neuronal degeneration in tauopathies: mechanisms and models. Biochim Biophys Acta 1739:331–354. doi:10.1016/j.bbadis.2004.06.018
Han DH, Na H-K, Choi WH, Lee JH, Kim YK, Won C, Lee S-H, Kim KP, Kuret J, Min D-H, Lee MJ (2014) Direct cellular delivery of human proteasomes to delay tau aggregation. Nat Commun 5:5633. doi:10.1038/ncomms6633
Lim S, Haque MM, Nam G, Ryoo N, Rhim H, Kim YK (2015) Monitoring of Intracellular Tau Aggregation Regulated by OGA/OGT Inhibitors. Int J Mol Sci 16:20212–20224. doi:10.3390/ijms160920212
Tak H, Haque MM, Kim MJ, Lee JH, Baik J-H, Kim Y, Kim DJ, Grailhe R, Kim YK (2013) Bimolecular fluorescence complementation; lighting-up tau-tau interaction in living cells. PLoS One 8:e81682. doi:10.1371/journal.pone.0081682
Kim D, Lim S, Haque MM, Ryoo N, Hong HS, Rhim H, Lee D-E, Chang Y-T, Lee J-S, Cheong E, Kim DJ, Kim YK (2015) Identification of disulfide cross-linked tau dimer responsible for tau propagation. Sci Rep 5:15231. doi:10.1038/srep15231
Strober, W. (2001) Trypan blue exclusion test of cell viability. Curr Protoc Immunol Ed John E Coligan Al Appendix 3, Appendix 3B. doi: 10.1002/0471142735.ima03bs21
Acknowledgment
This work was supported by the National Research Council of Science & Technology (NST) grant by the Korea government (MSIP) (No. CRC-15-04-KIST) and the Brain Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning(NRF-2016M3C7A1913845).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Lim, S., Kim, D., Kim, D.J., Kim, Y.K. (2017). Image-Based Analysis of Intracellular Tau Aggregation by Using Tau-BiFC Cell Model. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6598-4_22
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6598-4_22
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6596-0
Online ISBN: 978-1-4939-6598-4
eBook Packages: Springer Protocols