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Measurement of Dynamic F-Actin Changes During Exocytosis

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Exocytosis and Endocytosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1174))

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Abstract

Exocytosis requires the fusion of vesicle membrane to the cell membrane. It is tightly regulated and orchestrated in space and time by diverse cellular mechanisms. It has long been recognized that one of these mechanisms is an essential role played by the cytoskeleton. In particular, accumulating evidence shows that the F-actin network is engaged during the final stages of vesicle interactions with the cell membrane. Using a combination of methods it is now possible to gain insights into F-actin dynamics and reveal its role during exocytosis. Here, we describe the use of two-photon and confocal microscopy to visualize F-actin changes at the cell membrane during exocytosis.

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Acknowledgements

This work was supported by an Australian Research Council Grant DP110100642 and National Health and Medical Research Council Grants APP1002520and APP1020764.

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Authors and Affiliations

  1. School of Biomedical Sciences, University of Queensland, MacGregor Building (Building 64), 4072, St Lucia, QLD, Australia

    Peter Thorn

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  1. Peter Thorn
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Correspondence to Peter Thorn .

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Editors and Affiliations

  1. Department of Human and Molecular Genetics, VCU Institute of Molecular Medicine, Virginia Commonwealth University, Richmond, Virginia, USA

    Andrei I. Ivanov

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Thorn, P. (2014). Measurement of Dynamic F-Actin Changes During Exocytosis. In: Ivanov, A. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 1174. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0944-5_29

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  • DOI: https://doi.org/10.1007/978-1-4939-0944-5_29

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0943-8

  • Online ISBN: 978-1-4939-0944-5

  • eBook Packages: Springer Protocols

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