Exocytosis and Endocytosis

  • Andrei I. Ivanov

Part of the Methods in Molecular Biology book series (MIMB, volume 1174)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. An Overview of Methods for Analyzing Vesicular Trafficking

  3. Cell-Free and Biochemical Assays of Exocytosis and Endocytosis

  4. Imaging and Ultrastructural Analysis of Vesicle Trafficking in Cultured Cells

    1. Front Matter
      Pages 115-115
    2. Jean-Philippe Grossier, Bruno Goud, Kristine Schauer
      Pages 117-138
    3. Antje Schmidt, Burkhard Wiesner, Ralf Schülein, Anke Teichmann
      Pages 139-156
    4. Emily H. Stoops, Glen A. Farr, Michael Hull, Michael J. Caplan
      Pages 171-182
    5. Mickaël Lelek, Francesca Di Nunzio, Christophe Zimmer
      Pages 183-193
    6. Anton Khmelinskii, Michael Knop
      Pages 195-210
    7. Gaelle Boncompain, Franck Perez
      Pages 211-223

About this book

Introduction

Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane, and organelle trafficking in cultured cells, and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.

Keywords

biochemical assays cultured cells endocytosis exocytosis live cell imaging vesicular trafficking

Editors and affiliations

  • Andrei I. Ivanov
    • 1
  1. 1.Department of Human and Molecular GeneticsVCU Institute of Molecular Medicine, Virginia Commonwealth UniversityRichmondUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0944-5
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0943-8
  • Online ISBN 978-1-4939-0944-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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