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Metabolic Footprinting: Extracellular Metabolomic Analysis

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Book cover Pseudomonas Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1149))

Abstract

Uptake and excretion of nutrients is an integral part of a cell’s physiology. Using analytical chemistry techniques, metabolite uptake and excretion from the culture medium can be quantified. As cellular metabolism changes throughout growth, additional information is available if transient and growth phase-dependent changes are monitored. Here, we describe time-resolved metabolic footprinting (TReF), a technique which employs nuclear magnetic resonance spectroscopy and nonlinear curve fitting to understand and visualize metabolite utilization of P. aeruginosa.

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Acknowledgements

The authors thank Dr Olaf Beckonert and Dr Anthony Maher for technical assistance with NMR spectroscopy and Prof. Jeremy Nicholson and Prof. Elaine Holmes for support and access to facilities.

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Authors and Affiliations

  1. Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK

    Volker Behrends & Jacob G. Bundy

  2. Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, South Kensington Campus, London, UK

    Huw D. Williams

Authors
  1. Volker Behrends
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  2. Huw D. Williams
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  3. Jacob G. Bundy
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Corresponding author

Correspondence to Jacob G. Bundy .

Editor information

Editors and Affiliations

  1. MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  2. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

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© 2014 Springer Science+Business Media New York

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Behrends, V., Williams, H.D., Bundy, J.G. (2014). Metabolic Footprinting: Extracellular Metabolomic Analysis. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_23

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  • DOI: https://doi.org/10.1007/978-1-4939-0473-0_23

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-0472-3

  • Online ISBN: 978-1-4939-0473-0

  • eBook Packages: Springer Protocols

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