Abstract
The assembled sequence of the whole genome is a must nowadays in any industrially important species. The aim of this research program is to provide the industry and the community with a robust mink genome sequence assembly. It shall be complemented with the identification of widely distributed polymorphic single nucleotide polymorphisms (SNPs) organized into an array designed for undertaking linkage and association studies necessary for mapping, identifying and characterizing genes underlying any specific traits of interest in American mink. Genome assembly based on the sequences of individual clones from the mink Bacterial Artificial Chromosome (BAC) library would result in a draft genome of high quality and accuracy. We explore an alternative approach, called BAC-NGS (from next generation sequencing) that utilizes the BAC library as a solid backbone, complemented with shotgun sequences of the whole genome, for filling gaps for the final assembly. We tested different settings of the new approach in several pilot projects in order to assign the most efficacious approach to sequence assembly. Approximately 15% of the mink genome has so far been generated and assembled by means of this method. This sequencing project represents a long-term investment in the future mink fur industry and farming management, enhancing the development of applications in academic and industrial research.
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Anistoroaei, R., Christensen, K., Benkel, B. (2012). Sequencing of the mink genome: plans and perspectives. In: Larsen, P.F., et al. Proceedings of the Xth International Scientific Congress in fur animal production. Wageningen Academic Publishers, Wageningen. https://doi.org/10.3920/978-90-8686-760-8_31
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DOI: https://doi.org/10.3920/978-90-8686-760-8_31
Publisher Name: Wageningen Academic Publishers, Wageningen
Online ISBN: 978-90-8686-760-8
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