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Molecular Diagnostics in Coagulation

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Molecular Diagnostics

Abstract

Coagulation testing has evolved from the use of the manual tilt-tube clotting time in the early 1900’s to the use of molecular diagnostics today. Over the years, the measurement of a clot endpoint has been the basis of testing. Automated instruments have replaced the manual visual methods and allowed for precise measurements of the clot endpoint. In addition, immuno-logic and chromogenic methodology has also been adapted into the coagulation laboratory, therefore providing an additional antigenic and enzymatic perspective. Today, most modern automated coagulation instruments offer a single test platform that incorporates optic, immunologic, and chromogenic methods. The arrival of molecular diagnostics now adds another dimension for the evaluation of hemostatic defects.

Historically, coagulation testing was confined mostly to the screening or work-up of patients with bleeding disorders. The availability of routine laboratory tests for patients with thrombosis has slowly increased over the years and the demand for this testing continues to grow. Molecular diagnostics has found an important role in this area of coagulation disorders. DNA-based tests are available for detection of the Factor V Leiden mutation, the Prothrombin 20120A mutation, and the methyl-enetetrahydrofolate reductase mutation.

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  1. Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT

    Enrique Ballesteros MD

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  1. Enrique Ballesteros MD
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  1. Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USA

    William B. Coleman

  2. Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA

    Gregory J. Tsongalis

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Ballesteros, E. (2006). Molecular Diagnostics in Coagulation. In: Coleman, W.B., Tsongalis, G.J. (eds) Molecular Diagnostics. Humana Press. https://doi.org/10.1385/1-59259-928-1:311

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  • DOI: https://doi.org/10.1385/1-59259-928-1:311

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