Abstract
Biomaterials with functional properties are used to fabricate scaffolds for bone tissue engineering. Several of these materials can be derived from nature, processed and transformed into regenerative scaffolds and/or artificial matrices for applications in bone tissue repair or regeneration. In this chapter, we discuss the basic biology of bone development and the utilization of chitosan, hydroxyapatite and diatoms for BTE. The regenerative properties of Chitosan are desirable due to its close proximity with glycosaminoglycan—an extracellular matrix polysaccharide, which interacts with collagen fibers. Nano-hydroxyapatite is an inorganic component of natural bone matrix with osteoinductive properties. Diatoms are important source of biogenic silica and their high surface area, as well as nanoscopic pore structure make them desirable for delivery of biomolecules and reinforcing structural functions of three-dimensional scaffold matrices. Additionally, we discussed the methods used to fabricate the scaffolds for bone repair.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Boyce T, Edwards J, Scarborough N (1999) Allograft bone. The influence of processing on safety and performance. Orthop Clin North Am 30:571–581
Giannoudis PV, Dinopoulos H, Tsiridis E (2005) Bone substitutes: an update. Injury 36:S20–S27
Amini AR, Laurencin CT, Nukavarapu SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40:363–408
Summers BN, Eisenstein SM (1989) Donor site pain from the ilium. A complication of lumbar spine fusion. J Bone Joint Surg Br 71:677–680
Banwart JC, Asher MA, Hassanein RS (1995) Iliac crest bone graft harvest donor site morbidity. A statistical evaluation. Spine 20:1055–1060
Arrington ED, Smith WJ, Chambers HG et al (1996) Complications of iliac crest bone graft harvesting. Clin Orthop Relat Res 329:300–309
Ross N, Tacconi L, Miles JB (2000) Heterotopic bone formation causing recurrent donor site pain following iliac crest bone harvesting. Br J Neurosurg 14:476–479
Seiler JG 3rd, Johnson J (2000) Iliac crest autogenous bone grafting: donor site complications. J South Orthop Assoc 9:91–97
Skaggs DL, Samuelson MA, Hale JM et al (2000) Complications of posterior iliac crest bone grafting in spine surgery in children. Spine 25:2400–2402
Ehrler DM, Vaccaro AR (2000) The use of allograft bone in lumbar spine surgery. Clin Orthop Relat Res 371:38–45
Saiz E, Zimmermann EA, Lee JS et al (2013) Perspectives on the role of nanotechnology in bone tissue engineering. Dent Mater 29:103–115
Walmsley GG, McArdle A, Tevlin R et al (2015) Nanotechnology in bone tissue engineering. Nanomedicine 11:1253–1263
Rudman KE, Aspden RM, Meakin JR (2006) Compression or tension? The stress distribution in the proximal femur. Biomed Eng Online. https://doi.org/10.1186/1475-925x-5-12
Penido MGMG, Alon US (2012) Phosphate homeostasis and its role in bone health. Pediatr Nephrol 27:2039–2048
Song L (2017) Calcium and bone metabolism indices. Adv Clin Chem 82:1–46
Boskey AL, Spevak L, Paschalis E et al (2002) Osteopontin deficiency increases mineral content and mineral crystallinity in mouse bone. Calcif Tissue Int 71:145–154
Buckwalter JA, Glimcher MJ, Cooper RR et al (1996) Bone biology. I: structure, blood supply, cells, matrix, and mineralization. Instr Course Lect 45:371–386
Downey PA, Siegel MI (2006) Bone biology and the clinical implications for osteoporosis. Phys Ther 86:77–91
Clarke B (2008) Normal bone anatomy and physiology. Clin J Am Soc Nephrol 3:S131–S139
Florencio-Silva R, Sasso GR, Sasso-Cerri E et al (2015) Biology of bone tissue: structure, function, and factors that influence bone cells. Biomed Res Int 2015:421746. https://doi.org/10.1155/2015/421746
Capulli M, Paone R, Rucci N (2014) Osteoblast and osteocyte: games without frontiers. Arch Biochem Biophys 561:3–12
Grigoriadis AE, Heersche JN, Aubin JE (1988) Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone. J Cell Biol 106:2139–2151
Rochefort GY, Pallu S, Benhamou CL (2010) Osteocyte: the unrecognized side of bone tissue. Osteoporos Int 21:1457–1469
Dallas SL, Prideaux M, Bonewald LF (2013) The osteocyte: an endocrine cell … and more. Endocr Rev 34:658–690
Marks SC Jr, Popoff SN (1988) Bone cell biology: the regulation of development, structure, and function in the skeleton. Am J Anat 183:1–44
Kang JH, Ko HM, Moon JS et al (2014) Osteoprotegerin expressed by osteoclasts: an autoregulator of osteoclastogenesis. J Dent Res 93:1116–1123
Mosley JR (2000) Osteoporosis and bone functional adaptation: mechanobiological regulation of bone architecture in growing and adult bone, a review. J Rehabil Res Dev 37:189–199
Andersen TL, Sondergaard TE, Skorzynska KE et al (2009) A physical mechanism for coupling bone resorption and formation in adult human bone. Am J Pathol 174:239–247
Crockett JC, Mellis DJ, Scott DI et al (2011) New knowledge on critical osteoclast formation and activation pathways from study of rare genetic diseases of osteoclasts: focus on the RANK/RANKL axis. Osteoporos Int 22:1–20
Boyce BF, Hughes DE, Wright KR et al (1999) Recent advances in bone biology provide insight into the pathogenesis of bone diseases. Lab Invest 79:83–94
Yavropoulou MP, Yovos JG (2008) Osteoclastogenesis–current knowledge and future perspectives. J Musculoskelet Neuronal Interact 8:204–216
Takayanagi H (2007) Osteoimmunology: shared mechanisms and crosstalk between the immune and bone systems. Nat Rev Immunol 7:292–304
No H, Lee M (1995) Isolation of chitin from crab shell waste. J Korean Soc Food Nutrition 24:105–113
Bo M, Bavestrello G, Kurek D et al (2012) Isolation and identification of chitin in the black coral Parantipathes larix (Anthozoa: Cnidaria). Int J Biol Macromol 51:129–137
Wu T, Zivanovic S, Draughon FA et al (2004) Chitin and chitosan–value-added products from mushroom waste. J Agric Food Chem 52:7905–7910
Jayakumar R, Prabaharan M, Nair SV et al (2010) Novel chitin and chitosan nanofibers in biomedical applications. Biotechnol Adv 28:142–150
Croisier F, Jérôme C (2013) Chitosan-based biomaterials for tissue engineering. Eur Polym J 49:780–792
LogithKumar R, KeshavNarayan A, Dhivya S et al (2016) A review of chitosan and its derivatives in bone tissue engineering. Carbohydr Polym 151:172–188
Saharan V, Mehrotra A, Khatik R et al (2013) Synthesis of chitosan based nanoparticles and their in vitro evaluation against phytopathogenic fungi. Int J Biol Macromol 62:677–683
Ghadi A, Mahjoub S, Tabandeh F et al (2014) Synthesis and optimization of chitosan nanoparticles: potential applications in nanomedicine and biomedical engineering. Casp J Intern Med 5:156–161
Saravanan S, Sameera DK, Moorthi A (2013) Chitosan scaffolds containing chicken feather keratin nanoparticles for bone tissue engineering. Int J Biol Macromol 62:481–486
Lowe B, Venkatesan J, Anil S et al (2016) Preparation and characterization of chitosan-natural nano hydroxyapatite-fucoidan nanocomposites for bone tissue engineering. Int J Biol Macromol 93B:1479–1487
Manjubala I, Scheler S, Bössert J et al (2006) Mineralisation of chitosan scaffolds with nano-apatite formation by double diffusion technique. Acta Biomater 2:75–84
Zolghadri M, Saber-Samandari S, Ahmadi S et al (2019) Synthesis and characterization of porous cytocompatible scaffolds from polyvinyl alcohol-chitosan. Bull Mater Sci 42:35
Kim HL, Jung GY, Yoon JH et al (2015) Preparation and characterization of nano-sized hydroxyapatite/alginate/chitosan composite scaffolds for bone tissue engineering. Mater Sci Eng C Mater Biol Appl 54:20–25
Zhao J, Shen G, Liu C et al (2012) Enhanced healing of rat calvarial defects with sulfated chitosan-coated calcium-deficient hydroxyapatite/bone morphogenetic protein 2 scaffolds. Tissue Eng Part A 18:185–197
Shin SY, Park HN, Kim KH et al (2005) Biological evaluation of chitosan nanofiber membrane for guided bone regeneration. J Periodontol 76:1778–1784
Lee EJ, Shin DS, Kim HE et al (2009) Membrane of hybrid chitosan-silica xerogel for guided bone regeneration. Biomaterials 30:743–750
Pepla E, Besharat LK, Palaia G et al (2014) Nano-hydroxyapatite and its applications in preventive, restorative and regenerative dentistry: a review of literature. Ann Stomatol 5:108–114
Attia MS, Mohammed HM, Attia MG et al (2018) Histological and histomorphometric evaluation of hydroxyapatite-based biomaterials in surgically created defects around implants in dogs. J Periodontol. https://doi.org/10.1002/jper.17-0469
Bhardwaj VA, Deepika PC, Basavarajaiah S (2018) Zinc incorporated nano hydroxyapatite: a novel bone graft used for regeneration of intrabony defects. Contemp Clin Dent 9:427–433
Coelho TM, Nogueira ES, Steimacher A et al (2006) Characterization of natural nanostructured hydroxyapatite obtained from the bones of Brazilian river fish. J Appl Phys 100:094312. https://doi.org/10.1063/1.2369647
Ivankovic H, Tkalcec E, Orlic S et al (2010) Hydroxyapatite formation from cuttlefish bones: kinetics. J Mater Sci Mater Med 21:2711–2722
Venkatesan J, Qian ZJ, Ryu B et al (2011) A comparative study of thermal calcination and an alkaline hydrolysis method in the isolation of hydroxyapatite from Thunnus obesus bone. Biomed Mater 6:035003. https://doi.org/10.1088/1748-6041/6/3/035003
Boutinguiza M, Pou J, Comesaña R et al (2012) Biological hydroxyapatite obtained from fish bones. Mater Sci Eng C Mater Biol Appl 32:478–486
Piccirillo C, Silva MF, Pullar RC et al (2013) Extraction and characterisation of apatite- and tricalcium phosphate-based materials from cod fish bones. Mater Sci Eng C Mater Biol Appl 33:103–110
Venkatesan J, Lowe B, Manivasagan P et al (2015) Isolation and characterization of nano-hydroxyapatite from salmon fish bone. Materials 8:5426–5439
Pal A, Paul S, Choudhury AR et al (2017) Synthesis of hydroxyapatite from Lates calcarifer fish bone for biomedical applications. Mater Lett 203:89–92
Huang YC, Hsiao PC, Chai HJ (2011) Hydroxyapatite extracted from fish scale: effects on MG63 osteoblast-like cells. Ceram Int 37:1825–1831
Kongsri S, Janpradit K, Buapa K et al (2013) Nanocrystalline hydroxyapatite from fish scale waste: preparation, characterization and application for selenium adsorption in aqueous solution. Chem Eng J 215:522–532
Komalakrishna H, Shine JTG, Kundu B (2017) Low temperature development of nano-hydroxyapatite from Austromegabalanus psittacus, Star fish and Sea urchin. Mater Today Proc 4:11933–11938
Bigham-Sadegh A, Karimi I, Shadkhast M et al (2015) Hydroxyapatite and demineralized calf fetal growth plate effects on bone healing in rabbit model. J Orthop Traumatol 16:141–149
Kantharia N, Naik S, Apte S et al (2014) Nano-hydroxyapatite and its contemporary applications. Bone 34(1):71
Kattimani VS, Kondaka S, Lingamaneni KP (2016) Hydroxyapatite—past, present, and future in bone regeneration. Bone Tissue Regen Insights. https://doi.org/10.4137/btri.s36138
Yamada M, Ueno T, Tsukimura N (2012) Bone integration capability of nanopolymorphic crystalline hydroxyapatite coated on titanium implants. Int J Nanomedicine 7:859–873
Singh VP, Nayak DG, Uppoor AS et al (2012) Clinical and radiographic evaluation of nano-crystalline hydroxyapatite bone graft (Sybograf) in combination with bioresorbable collagen membrane (Periocol) in periodontal intrabony defects. Dent Res J 9:60–67
Qu Y, Wang P, Man Y et al (2010) Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane. Int J Nanomedicine 5:429–435
Xiong Y, Ren C, Zhang B et al (2014) Analyzing the behavior of a porous nano-hydroxyapatite/polyamide 66 (n-HA/PA66) composite for healing of bone defects. Int J Nanomedicine 9:485–494
Wang Y, Cai J, Jiang Y et al (2013) Preparation of biosilica structures from frustules of diatoms and their applications: current state and perspectives. Appl Microbiol Biotechnol 97:453–460
Venkatesan J, Lowe B, Kim SE (2015) Application of diatom biosilica in drug delivery. In: Kim SE (ed) Handbook of marine microalgae. Academic Press, Massachusetts, pp 245–254
Round FE, Crawford RM, Mann DG (1990) The diatoms. Biology and morphology of the genera. Cambridge University Press, Cambridge
Battarbee RW, Jones VJ, Flower RJ et al (2001) Diatoms. In: Smol JP, Birk HJB, Last WM (eds) Tracking environmental change using lake sediments, vol 3. Terrestrial, algal, and siliceous indicators. Kluwer Academic Publishers, Dordrecht, pp 155–202
Zhou H, Fan T, Zhang D (2011) Biotemplated materials for sustainable energy and environment: current status and challenges. Chemsuschem 4:1344–1387
Tamburaci S, Tihminlioglu F (2017) Diatomite reinforced chitosan composite membrane as potential scaffold for guided bone regeneration. Mater Sci Eng C Mater Biol Appl 80:222–231
Goren R, Baykara T, Marsoglu M (2002) A study on the purification of diatomite in hydrochloric acid. Scand J Metall 31:115–119
Lettieri S, Setaro A, De Stefano L et al (2008) The gas-detection properties of light-emitting diatoms. Adv Funct Mater 18:1257–1264
Jeffryes C, Solanki R, Rangineni Y et al (2008) Electroluminescence and photoluminescence from nanostructured diatom frustules containing metabolically inserted germanium. Adv Mater 20:2633–2637
Lin KC, Kunduru V, Bothara M et al (2010) Biogenic nanoporous silica-based sensor for enhanced electrochemical detection of cardiovascular biomarkers proteins. Biosens Bioelectron 25:2336–2342
De Stefano L, Rotiroti L, De Stefano M et al (2009) Marine diatoms as optical biosensors. Biosens Bioelectron 24:1580–1584
Noll F, Sumper M, Hampp N (2002) Nanostructure of diatom silica surfaces and of biomimetic analogues. Nano Lett 2:91–95
Leynaert A, Fardel C, Beker B et al (2018) Diatom frustules nanostructure in pelagic and benthic environments. Silicon 10:2701–2709
Martin-Jézéquel V, Hildebrand M, Brzezinski MA (2000) Silicon metabolism in diatoms: implications for growth. J Phycol 36:821–840
Falciatore A, Bowler C (2002) Revealing the molecular secrets of marine diatoms. Annu Rev Plant Biol 53:109–130
Jan JS, Chen PS, Hsieh PL et al (2012) Silicification of genipin-cross-linked polypeptide hydrogels toward biohybrid materials and mesoporous oxides. ACS Appl Mater Interfaces 4:6865–6874
De Tommasi E, Rea I, De Stefano L et al (2013) Optics with diatoms: towards efficient, bioinspired photonic devices at the micro-scale. In: Ferraro P, RitschMarte M, Grilli S, Stifter D (eds) Optical methods for inspection, characterization, and imaging of biomaterials. Proceedings of SPIE, vol 8792. SPIE-International Society of Optical Engineering, Washington
Di Caprio G, Coppola G, De Stefano L et al (2014) Shedding light on diatom photonics by means of digital holography. J Biophotonics 7:341–350
Dixit SS, Smol JP, Kingston JC et al (1992) Diatoms—powerful indicators of environmental change. Environ Sci Technol 26:22–33
Douglas M, Smol J (1999) Freshwater diatoms as indicators of environmental change in the high Arctic. In: Stoermer E, Smol J (eds) The diatoms: applications for the environmental and earth sciences. Cambridge University Press, Cambridge, pp 227–244
Potapova M, Charles DF (2007) Diatom metrics for monitoring eutrophication in rivers of the United States. Ecol Indic 7:48–70
Lorenzen J, Larsen LH, Kjaer T et al (1998) Biosensor determination of the microscale distribution of nitrate, nitrate assimilation, nitrification, and denitrification in a diatom-inhabited freshwater sediment. Appl Environ Microbiol 64:3264–3269
Jeffryes C, Campbell J Li H et al (2011) The potential of diatom nanobiotechnology for applications in solar cells, batteries, and electroluminescent devices. Energy Environ Sci 4:3930–3941
Ge M, Fang X, Rong J et al (2013) Review of porous silicon preparation and its application for lithium-ion battery anodes. Nanotechnology 24:422001. https://doi.org/10.1088/0957-4484/24/42/422001
Le TDH, Liaudanskaya V, Bonani W et al (2018) Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications. J Tissue Eng Regen Med 12:89–97
Losic D, Mitchell JG, Voelcker NH (2009) Diatomaceous lessons in nanotechnology and advanced materials. Adv Mater 21:2947–2958
Le TDH, Bonani W, Speranza G et al (2016) Processing and characterization of diatom nanoparticles and microparticles as potential source of silicon for bone tissue engineering. Mater Sci Eng C Mater Biol Appl 59:471–479
Walsh PJ, Clarke SA, Julius M et al (2017) Exploratory testing of diatom silica to map the role of material attributes on cell fate. Sci Rep 7:14138. https://doi.org/10.1038/s41598-017-13285-4
Kimura K, Tomaru Y (2013) A unique method for culturing diatoms on agar plates. Plankton Benthos Res 8:46–48
Sanjay K, Nagendra PM, Anupama S et al (2013) Isolation of diatom Navicula cryptocephala and characterization of oil extracted for biodiesel production. Afr J Environ Sci Tech 7:41–48
Gordon R, Parkinson J (2005) Potential roles for diatomists in nanotechnology. J Nanosci Nanotechnol 5:35–40
Whitton B, Ellwood N, Kawecka B (2009) Biology of the freshwater diatom Didymosphenia: a review. Hydrobiologia 630:1–37
Acknowledgements
This project in part was supported by the Australian Government Research Training Program Scholarship, The University of Queensland; The UQDVCR (610709 to Qingsong Ye); MGH-OMFS Education Research Fund; The Lynn Foundation; The Jean Foundation; The Walter C. Guralnick Fund.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Lowe, B., Guastaldi, F., Müller, ML., Gootkind, F., Troulis, M.J., Ye, Q. (2019). Nanobiomaterials for Bone Tissue Engineering. In: Choi, A., Ben-Nissan, B. (eds) Marine-Derived Biomaterials for Tissue Engineering Applications. Springer Series in Biomaterials Science and Engineering, vol 14. Springer, Singapore. https://doi.org/10.1007/978-981-13-8855-2_4
Download citation
DOI: https://doi.org/10.1007/978-981-13-8855-2_4
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-8854-5
Online ISBN: 978-981-13-8855-2
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)