Abstract
The production of doubled haploid (DH) barley (Hordeum vulgare L.) plants has proven to be a highly valuable tool for plant breeding, allowing the release of a high number of new cultivars (Devaux et al., 1996). Doubled haploids have also been fundamental for genetic analysis, linkage maps production and QTL analysis in barley. Several anther culture protocols have been established for an efficient production of DH lines by different public institutions and private companies. However, the low embryogenic capacity and the high albinism rate of some genotypes are still open fields for further improvements in these protocols. To be successfully used in a breeding program, any particular protocol should produce a large number of DH lines from all the genotypes. During the last years, our aim has been focused on the establishment of an efficient protocol for the production of doubled haploid plants from a wide range of genotypes, as well as from F1 crosses of putative great agronomic performance. The results achieved over five years (1993–1997) in our laboratory resulted in a gradual increase in the numbers of green plants per 100 anthers (from 6.3 up to 17.4), as well as DH fertile lines per 100 anthers (from 1.2 to 10.3). Major changes of the initial protocol have been the increase of mannitol concentration in the pretreatment medium from 0.3 to 1.0 M, the introduction of Ficoll in the induction medium, the optimization of the regeneration medium composition, and the procedure for transferring the plants to soil (Cistué et al., 1994, 1999; Castillo et al., 2000).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Castillo, A. M., M. P. Valles and L. Cistue, 2000. Comparison of anther and isolated microspore cultures in barley. Effects of culture density and regeneration medium. Euphytica 113: 1–8.
Cistue, L., A. Ramos and A.M. Castillo, 1999. Influence of anther pretreatment and culture medium composition on the production of barley doubled haploids from model and low responding cultivars. Plant Cell Tiss.Org.Cult. 55: 159–166.
Cistué, L., A. Ramos, A.M. Castillo and I. Romagosa, 1994. Production of large number of doubled haploid plants from barley anthers pretreated with high concentrations of mannitol Plant Cell Rep. 13: 709–712.
Devaux, P., M. Zivy, A. Kilian and A. Kleinhofs, 1996. Doubled haploids in barley. In: Proc. V International Oat Conference and VII International Barley Genetics Symposium. Scoles, G. and B. Rossnagel (Eds.) Univ. of Saskatchewen, Saskatoon, pp. 213–222.
Hunter, C.P., 1988. Plant regeneration from microspores of barley, Hordeum vulgare L. Ph.D. Thesis. Wye College, Univ. of London, London.
Jensen, C.J., 1977. Monoploid production by chromosome elimination. In: Applied and Fundamental aspects of Plant Cell, Tissue and Organ Culture. Reinert, J. and Y.P.S. Bajaj (Eds.) Springer-Verlag, Berlin, pp. 299–330.
Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol.Plant. 15: 473–497.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media New York
About this chapter
Cite this chapter
Cistué, L., Vallés, M.P., Echávarri, B., Sanz, J.M., Castillo, A. (2003). Barley anther culture. In: Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I. (eds) Doubled Haploid Production in Crop Plants. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1293-4_5
Download citation
DOI: https://doi.org/10.1007/978-94-017-1293-4_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-6393-9
Online ISBN: 978-94-017-1293-4
eBook Packages: Springer Book Archive