Doubled Haploid Production in Crop Plants

A Manual

  • M. Maluszynski
  • K. J. Kasha
  • B. P. Forster
  • I. Szarejko

Table of contents

  1. Front Matter
    Pages N1-xlvi
  2. Production of doubled haploids in crop plants. An introduction

  3. Protocols for major crops

    1. Barley

      1. P. Devaux
        Pages 15-19
      2. C. Jacquard, G. Wojnarowiez, C. Clément
        Pages 21-27
      3. L. Cistué, M. P. Vallés, B. Echávarri, J. M. Sanz, A. Castillo
        Pages 29-34
      4. K. J. Kasha, E. Simion, R. Oro, Y. S. Shim
        Pages 43-47
    2. Wheat

      1. J. Pauk, R. Mihály, M. Puolimatka
        Pages 59-64
      2. S. Tuvesson, R. von Post, A. Ljungberg
        Pages 71-76
      3. K. J. Kasha, E. Simion, M. Miner, J. Letarte, T. C. Hu
        Pages 77-81
    3. Maize

    4. Rice indica/japanica

    5. Triticale

    6. Rye

      1. S. Immonen, T. Tenhola-Roininen
        Pages 141-149
      2. S. Pulli, Y.-D. Guo
        Pages 151-154
    7. Oat

      1. H. W. Rines
        Pages 155-159
    8. Durum wheat

    9. Timothy

    10. Ryegrass and other grasses

    11. Rapeseed

    12. Broccoli

      1. J. C. da Silva Dias
        Pages 195-204
    13. Other Brassicas

    14. Tobacco

      1. A. Touraev, E. Heberle-Bors
        Pages 223-228
    15. Potato

    16. Linseed/flax

    17. Sugar beet

      1. E. Wremerth Weich, M. W. Levall
        Pages 255-263
    18. Asparagus

      1. D. J. Wolyn, B. Nichols
        Pages 265-273
    19. Onion

    20. Apple

      1. M. Höfer
        Pages 287-292
    21. Aspen

      1. S. B. Andersen
        Pages 293-296
    22. Cork oak

      1. M. A. Bueno, J. A. Manzanera
        Pages 297-301
    23. Citrus

      1. M. A. Germanà
        Pages 303-307
  4. Published doubled haploid protocols in plant species

    1. M. Maluszynski, K. J. Kasha, I. Szarejko
      Pages 309-335
  5. Application of doubled haploid production techniques

    1. W. T. B. Thomas, B. P. Forster, B. Gertsson
      Pages 337-349
    2. I. Szarejko
      Pages 351-361
    3. Y. S. Shim, K. J. Kasha
      Pages 363-366

About this book


The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes. This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for many crops including some woody tree species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations. For many years, both the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) have supported and coordinated research that focuses on development of more efficient doubled haploid production methods and their applications in breeding of new varieties and basic research through their Plant Breeding and Genetics Section of the Joint F AO/IAEA Division of Nuclear Techniques in Food and Agriculture. The first F AO/IAEA scientific network (Coordinated Research Programme - CRP) dealing with doubled haploids was initiated by the Plant Breeding and Genetics Section in 1986.


Brassica napus L. Embryo Hordeum vulgare L. Linum usitatissimum L. Triticale Triticosecale Wittm. Triticum aestivum L. Zea mays L. breeding crop improvement crop plants genetics maize plant breeding wheat

Editors and affiliations

  • M. Maluszynski
    • 1
  • K. J. Kasha
    • 2
  • B. P. Forster
    • 3
  • I. Szarejko
    • 4
  1. 1.Joint FAO/IAEA Division of Nuclear Technique in Food and AgricultureViennaAustria
  2. 2.Department of Plant AgricultureUniversity of GuelphGuelphCanada
  3. 3.Scottish Crop Research InstituteDundeeUK
  4. 4.Department of GeneticsUniversity of SilesiaKatowicePoland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 2003
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-90-481-6393-9
  • Online ISBN 978-94-017-1293-4
  • Buy this book on publisher's site
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