Radial immunodiffusion and rocket Immunoelectrophoresis

Abstract

Specific protein concentrations may be measured in gel media by two techniques: single radial immunodiffusion and rocket Immunoelectrophoresis. The former method is called ‘single’ to distinguish it from the qualitative Ouchter-lony or ‘double diffusion’ methods, and ‘radial’ to contrast it with the earlier ‘linear diffusion’ technique of Oudin¹. Rocket Immunoelectrophoresis is also called ‘electroimmunoassay’. The original versions of these techniques were described respectively by Mancini, Carbonara and Heremans in 1965², and Laurell in 1966³. Verbruggen⁴ has recently published an extensive review of quantitative immunoelectrophoretic methods.