Abstract
From other chapters it will be evident that the molecular biologist often needs to separate the components of a complex mixture of DNA molecules either analytically or preparatively. Although it is sometimes possible to use density or affinity techniques to separate DNA molecules on the basis of their base compositions, such an approach cannot be used with complex mixtures or when, as is often the case, different DNAs have similar base compositions. Separations based on size differences are far more generally useful. The identification of restriction fragments containing a particular sequence of bases (e.g. a particular gene) depends on the separation of DNA fragments according to size (Chapter 15), as does the mapping of the position of such a sequence in the intact DNA. Separated fragments can be recovered and then replicated indefinitely by cloning (Chapters 11, 12 and 13). The sequencing of DNA also relies on the ability to resolve nucleic acids by size (Chapter 16).
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© 1983 John M. Walker and Wim Gaastra
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Boffey, S.A. (1983). Gel Electrophoresis of DNA. In: Walker, J.M., Gaastra, W. (eds) Techniques in Molecular Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-6563-1_14
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DOI: https://doi.org/10.1007/978-94-011-6563-1_14
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