Techniques in Molecular Biology

  • John M. Walker
  • Wim Gaastra

Table of contents

  1. Front Matter
    Pages 1-9
  2. Bryan John Smith, Robert H. Nicolas
    Pages 25-48
  3. Keith Gooderham
    Pages 49-61
  4. John M. Walker, Elaine L. V. Mayes
    Pages 63-85
  5. John M. Walker
    Pages 87-112
  6. Robert J. Slater
    Pages 113-133
  7. Don Grierson, Jim Speirs
    Pages 135-158
  8. Christopher G. P. Mathew
    Pages 159-166
  9. Brian G. Forde
    Pages 167-183
  10. Stephen A. Boffey
    Pages 185-195
  11. Frits R. Mooi, Wim Gaastra
    Pages 197-219
  12. Stephen A. Boffey
    Pages 257-271
  13. Christopher G. P. Mathew
    Pages 273-285
  14. Wim Gaastra, Bauke Oudega
    Pages 287-307
  15. Jan van Embden
    Pages 309-321
  16. Back Matter
    Pages 322-333

About this book


The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.


DNA RNA Southern Blot Termination biology chemistry molecular biology protein translation

Editors and affiliations

  • John M. Walker
    • 1
  • Wim Gaastra
    • 2
  1. 1.Department of Biological SciencesThe Hatfield PolytechnicHatfield, HertsEngland
  2. 2.Department of Applied Microbial GeneticsTechnical University of DenmarkLyngbyDenmark

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 1983
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-7099-2755-6
  • Online ISBN 978-94-011-6563-1
  • Buy this book on publisher's site
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