Abstract
Immunization with the zona pellucida (ZP) antigens elicits antibodies that confer anti-fertility effect, but also a CD4 T cell response that induces autoimmune ovarian disease. The complication of antoimmune ovarian disease induction has been overcome by the use of the chimeric peptide (CP) ZP vaccine. The CP is composed of a linear native B cell epitope of ZP3 or ZP2 in tandem with a foreign T cell epitope, and it induces antibody response to ZP without a concomitant T cell response to the ZP antigen. In this study, we describe our experience in the design of ZP CP vaccine, summarize the structure and immune responses to the CP with the murine and primate ZP3 and ZP2 B cell epitopes, and demonstrate the anti-fertility effect of CP vaccination. The result suggests that the multiple subunit CP vaccine design will likely lead to the formulation of a safe and effective ZP vaccine.p
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Gamete interaction, prospects for immunocontraception. Alexander N.J., Griffin D., Spieler J.M., Waites G.M.H. (eds.), New York: Wiley-Liss; 1990.
Primakoff P., Lathrop W., Woolman L., Cowan A., Myles D. Fully effective contraception in female guinea pigs immunized with the sperm protein PH-20. Nature 1988; 335: 543–546.
East I.J., Dean J. Monoclonal antibodies as probes of the distribution of ZP-2, the major sulfated glycoprotein of the murine zona pellucida. J. Cell. Biol. 1984; 98: 795–800.
East I.J., Mattison D.R., Dean J. Monoclonal antibodies to the major protein of the murine zona pellucida: effects on fertilization and early development. Dev. Biol. 1984; 104: 49–56.
Dunbar B.S. Ovarian antigens and infertility. Am. J. Reprod. Immunol 1997; 21: 28–31.
Mahi-Brown C.A., Yanagimachi R., Hoffman J.C., Huang T.T., Jr. Fertility control in the bitch by active immunization with porcine zonae pellucidae: use of different adjuvant and patterns of estradiol and progesterone levels in estrous cycles. Biol. Reprod. 1985; 32: 761–772.
Sacco A.G. Zona pellucida: current status as a candidate antigen for contraceptive vaccine development. Am. J. Reprod. Immunol. Microbiol. 1987; 15: 122–130.
Paterson M., Koothan P.T., Morris K.D., O’Bryne K.T., Braude P., Williams A., Aitken R.J. Analysis of contraceptive potential of antibodies against native and deglycosylated porcine ZP3 in vivo and in vitro. Biol. Reprod. 1992; 46: 523–534.
Paterson M., Wilson M.R., Vanduin M., Aitken R.J. Evaluation of zona pellucida antigens as potential candidates for immunocontraception. J. Reprod. Fertil. Suppl. 1996; 50: 175–182.
Rhim S.H., Millar S.E., Robey F., Luo A-M., Lou. Y-H., Yule T., Allen P., Dean J., Tung KSK. Autoimmune disease of the ovary induced by a ZP3 peptide from the mouse zona pellucida. J. Clin. Invest 1992; 89: 28–35.
Lou Y.H., Park K.K., Agersborg S.S., Tung K.S.K. Binding of autoantibody to an ovarian tissue antigen directs targeting of T cell mediated oophoritis. 1996; Serono Symposium: XIth Ovarian workshop: Ovarian cell growth, Apoptosis and Cancer, Abstract no. 31.
Bagavant H., Adams S.,Tarranova P., Chang A., Kraemer F.W., Lou Y.H., Kasai K., Luo A.M., Tung K.S.K. Autoimmune ovarian inflammation triggered by pro-inflammatory (Thl) T cells is compatible with normal ovarian function. Biol. Reprod. 1999 (in press).
Lou Y.H., Ang. J., Thai H., McElveen F, Tung K.S.K. A zone pellucida 3 peptide vaccine induces antibodies and reversible infertility without ovarian pathology. J. Immunol. 1995; 155: 2715–2720.
Millar S.E., Chamow S.M., Baur A.W., Oliver C., Robey F., Dean J. Vaccination with synthetic zona pellucida peptide produces long-term contraception in female mice. Science 1989; 246: 935–938.
Wei. S., Lou Y.H., Dean J., Tung K.S.K. A contraceptive peptide vaccine targeting ZP2 of the mouse zona pellucida. Biol. Reprod. 1999; 60: 900–907.
Lou. Y.H., Tung K.S.K. T cell peptide of a self protein elicits autoantibody to the protein antigen: implications for specificity and pathogenetic role of antibody in autoimmunity. J. Immunol 1993; 151: 5790–5799.
Lou., Y.H., McElveen M.F., Garza K.M., Tung K.S.K. Rapid induction of autoantibodies by endogenous ovarian antigens and activated T cells: Implication in autoimmune disease pathogenesis and B cell tolerance. J. Immunol. 1996; 156: 3535–3540.
Mahi-Brown C., Moran F. Response of cynomolgus macaques to immunization against a synthetic peptide form the human zona pellucida. J. Med. Primatol 1995; 24: 258–270.
Bagavant H., Fusi F.M., Baisch J., Kurth B., David C.S., Tung K.S.K. Immunogenicity and contraceptive potential of a human zona pellucida 3 peptide vaccine. Biol. Reprod. 1997; 56: 764–770.
Chen J.S., Lorenz R.G., Goldberg J., Allen P. Identification and characterization of a T cell-inducing epitope of bovine ribonuclease that can be restricted by multiple class II molecules. J. Immunol. 1991; 147: 3672–3678.
Good M.F., Maloy W.L., Lunde M.N., Margalit H., Cornette J.L., Smith G.L., Miller L.H., Berzofsky J.A. Construction of synthetic immunogen: use of new T-helper epitope on malaria circumsporozoite protein. Science 1987; 235: 1059–1062.
Wang Y., Smith J.A., Kamradt T., Gefter M., Perkins D.L. Silencing of immunodominant epitopes by contiguous sequences in complex synthetic peptides. Cell. Immunol. 1992; 143: 284–297.
Bhardwaj V., Kumar V., Geysen H.M., Sercarz E.E. Subjugation of dominant immunogenic determinants within a chimeric peptide. Eur. J. Immunol. 1992; 22: 2009–2016.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Tung, K.S.K., Lou, Y., Bagavant, H. (1999). Zona Pellucida Chimeric Peptide Vaccine. In: Gupta, S.K. (eds) Reproductive Immunology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4197-0_32
Download citation
DOI: https://doi.org/10.1007/978-94-011-4197-0_32
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5825-4
Online ISBN: 978-94-011-4197-0
eBook Packages: Springer Book Archive