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Quantitative Histochemical and Cytochemical Assays

  • Chapter
Histochemical and Immunohistochemical Techniques

Abstract

Histochemistry and cytochemistry are important tools for the analysis of toxic effects of xenobiotics because changes in metabolic functions can be directly related to the morphology of a tissue or the type of cell involved. A toxin may affect one type of cell which represents only a small percentage of the total volume of a tissue. Such effects will be missed when biochemical analysis is performed on a homogenate. However, localization of metabolic functions or cell constituents in itself is not sufficient and is only part of the picture besides quantitative information of the chemical processes involved. The overall goal of histochemistry and cytochemistry is to achieve understanding of the localization and the quantification of constituents and metabolic functions of tissues and cells and their changes under various conditions. Quantitative histochemistry and cytochemistry fill the gap between biochemical analysis (which provides virtually no topographic information) and histochemical or cytochemical localization which yields little quantitative information. The goal of quantitative histochemistry and cytochemistry is to increase localization of what can be quantified and to increase quantification of what can be localized (Glick, 1977).

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Authors
  1. Cornelis J. F. Van Noorden
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  2. Reinhart Gossrau
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© 1991 Peter Bach and John Baker

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Van Noorden, C.J.F., Gossrau, R. (1991). Quantitative Histochemical and Cytochemical Assays. In: Histochemical and Immunohistochemical Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3094-3_6

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  • DOI: https://doi.org/10.1007/978-94-011-3094-3_6

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5370-9

  • Online ISBN: 978-94-011-3094-3

  • eBook Packages: Springer Book Archive

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