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Histochemical and Immunohistochemical Techniques

Applications to pharmacology and toxicology

  • Peter H. Bach
  • John Baker

Table of contents

  1. Front Matter
    Pages i-xii
  2. M. G. Ormerod, J. A. Green
    Pages 103-118
  3. Cornelis J. F. Van Noorden, Reinhart Gossrau
    Pages 119-145
  4. David Hopwood
    Pages 147-165
  5. Peter Bannasch, Hans Jörg Hacker, Fritz Klimek, Doris Mayer, Heide Zerban
    Pages 187-223
  6. Neill J. Gregg, Martin F. Wilks, Peter H. Bach
    Pages 225-253
  7. Back Matter
    Pages 275-289

About this book

Introduction

Toxicological and pharmacological effects arise when chemicals interact with biophysiological functions in discrete cell types. There is a continuing need to screen novel compounds for their potential therapeutic effects, and once these have been "discovered" to understand their molecular actions, as the basis of using such compounds safely and for rational drug design. Pharmacology now uses all of the sophisticated molecular research techniques that are available for the development of safer and more efficacious drugs. Histochemistry has been usefully applied to developing new drugs (and assessing chemical safley) and is potentially cost effective. The need to test novel substances for their potential adverse effects has raised many questions. Toxicological pathology has moved away from the cataloging of lesions towards understanding the basis of the events that underly cell injury, especially for those secondary consequences of chemical injury that lead to malignancy and chronic disease. The focal nature of toxicologic lesions de­ mands the use of microtechniques to provide data to help understand these questions. Histochemistry is under-utilized, but offers one of the key ap­ proaches necessary to address the problem of understanding interactions between a cell population and a chemical, the modulation of cellular biochem­ istryor the presence of a lesion in a test animal can be rationalised in terms of species differences that have no relevance to man as opposed to those that are of clinical significance or represent a warning of dire consequences to man.

Keywords

cell pathology tissue toxicology

Authors and affiliations

  • Peter H. Bach
    • 1
  • John Baker
    • 2
  1. 1.The Nephrotoxicity Research Group, School of SciencePolytechnic of East LondonLondonUK
  2. 2.CIBA-GEIGY PharmaceuticalsWest SussexUK

Bibliographic information

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