Skip to main content
SpringerLink
Log in

Whole-Body Autoradiography of Pharmaceuticals and Chemicals

  • Chapter
Histochemical and Immunohistochemical Techniques

Abstract

Autoradiography has made important contributions to the study of cells and tissues in such diverse fields as basic medical sciences, pharmaceutical research, toxicology, immunology, plant sciences, etc. In pharmaceutical sciences, autoradiography has become a powerful research tool since it allows tracing of labelled molecules in the body or in cells. Thus, autoradiography is carried out every time the fate of a compound has to be elucidated. As conventional histological methods involve the use of solvents for preparing samples, they may extract or cause the translocation of labelled compounds during preparation. For this reason, it is common to perform studies using frozen specimens to obtain sections by cryomicrotomy. This chapter covers the study of xenobiotics in the body of animals, namely whole-body autoradiography.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • AKEL, G., BÉNARD, P., CANAL, P. and SOULA, G. (1986), Distribution and tumor penetration properties of a radiosensitizer 2(14C)misonidazole (Ro 07–0582), in mice and rats as studied by whole-body autoradiography. Cancer Chemother. Pharmacol., 17, 121–126.

    Article  PubMed  CAS  Google Scholar 

  • AKESSON, C. (1974), Autoradiographic studies on the distribution of 14C-2-ethanol and its non-volatile metabolites in the pregnant mouse. Arch. Int. Pharm. Ther., 109, 296–304.

    Google Scholar 

  • AKESSON C. and ULLBERG, S. (1972), Autoradiography of ethanol and histochemistry of alcohol dehydrogenase. Acta Pharmacol. Toxicol., 31, (Suppl. 1), 105.

    Google Scholar 

  • APPELGREN, L.E. (1967), Sites of steroid hormone formation. Autoradiographic studies using labelled precursors. Acta Physiol. Scand., Suppl. 301, 1–107.

    Google Scholar 

  • APPELGREN, L.E. (1969), Histochemical demonstration of drug interference with progesterone synthesis. J. Reprod Fertil., 19, 185–186.

    Article  PubMed  CAS  Google Scholar 

  • APPELGREN, L.E. (1971), Selective localization of labelled testosterone after in vitro incubation. Proc. Roy. Microsc. Soc., 6, 147–151.

    Google Scholar 

  • APPELGREN, L.E. (1983), Drug disposition in the fetus. In: Veterinary Parmacology and Toxicology, (Ruckebusch, Y. Toutain, P.L., and Koritz, G.D., eds.), Chap. 3. 39–55, MTP Press, Boston.

    Google Scholar 

  • BENAKIS, A. (1977), Specific localization of drugs at the hypophysis level and side effects: the case of neuroleptics. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 100–101.

    Google Scholar 

  • BENAKIS, A. and REY, C. (1976), Etude autoradiographique du Sulpiride 14C chez la souris et le rat. Localisation spĂ©cifique au niveau de l’hypophyse du rat après administration unique et administrations rĂ©pĂ©tĂ©es. J. Pharmacol. (Paris), 7, 367–378.

    CAS  Google Scholar 

  • BENAKIS, A., BROWN, J.P.H. and BÉNARD, P. (1984), Autoradiographic study of 14C-Sulpiride in monkey. Europ. J. Drug Metabolism Pharmacokin., 9, 365–370.

    Article  CAS  Google Scholar 

  • BENAKIS, A., PONGIS, M.A., SUGNAUX, F.R. and GLASSON, B. (1976), Localization, distribution, elimination and metabolism of 14C-Sulpiride in rats. Europ. J. Drug Metabolism Pharmacokin., 1, 51–52.

    Article  CAS  Google Scholar 

  • BÉNARD, P. (1978), Contribution a l’étude par autoradiographie macroscopique du mĂ©tabolisme de dĂ©rivĂ©s minĂ©raux du soufre et du sĂ©lĂ©nium chez diffĂ©rentes espèces animales. Thèse Doctorat de 3ème Cycle, No. 2199, Toulouse, France.

    Google Scholar 

  • BÉNARD, P. and DURAND, S. (1980), Distribution of radioactivity in rats injected with L-14C methionine as studied by whole-body autoradiography. Ann. Vet. Res., 11, 201–208.

    Google Scholar 

  • BÉNARD, P. and RICO, A.G. (1985), Whole-body autoradiography as a tool for studying milk excretion of xenobiotics: an alternative to pharmacokinetic studies. J. Pharm. Sci., 4, (Suppl. 1), 311–319.

    Google Scholar 

  • BÉNARD, P., RICO, A.G., BRAUN, J.P. and BURGAT-SACAZE, V. (1977), New approach to the visualization of recetors by autoradiography. Acta Pharmacol. Toxicol., 41, Suppl. 1, 20–21.

    Google Scholar 

  • BÉNARD, P., CAMBON, C. and DECLUME, C. (1979), Passage of radioactivity into the milk of rats treated with 14C-Carbaryl. Toxicol. Letters, 4, 149–153.

    Article  Google Scholar 

  • BÉNARD, P., JEANNIOT, J.P., BRAUN, J.P., BURGAT, V. and RICO, A.G. (1981), Etude par autoradiographie macroscopique de la distribution de la radioactivitĂ© chez des singes macaques ayant reçu du chlorhydrate de Minaprine. Concours MĂ©d., 77, (Suppl. 24), 24–27.

    Google Scholar 

  • BÉNARD, P., BRAUN, J.P., MAQUET, J.P., BURGAT, V. and RICO, A.G. (1981), Nephrotoxicity of cis-DDP in the rat. In: Organ-directed Toxicity. Chemical Indices and Mechanisms, (Brown, S.S. and Davies, D.S., eds.), pp. 75–79. Pergamon Pess, Oxford.

    Google Scholar 

  • BÉNARD, P., DESPLANCHES, G., MACQUET, J.P. and SIMON, J. (1983), Whole body autoradiographic study of the distribution of195mPt in healthy and tumor-bearing mice treated with labelled cisplatin. Cancer Treat. Rep., 67, 457–466.

    PubMed  Google Scholar 

  • BÉNARD, P., RICO, A.G. and BURGAT, V. (1985), Application of whole-body autoradiography in toxicology. C.R.C. Crit. Rev. Toxicol., 15, 181–215.

    Article  Google Scholar 

  • BÉNARD, P., PUOZZO, C., ROUX, C. and RICO, A.G. (1989), Transplacental passage and milk excretion of radioactivity in laboratory animals dosed with 14C-F 2207: a quantitative whole-body autoradiographic study. In: Synthesis and Applications of Isotopically Labelled Compounds. (Baillie T.A. and Jones J.R. eds.), pp. 637–640. Elsevier, Amsterdam.

    Google Scholar 

  • BENGTSSON, G. (1963), Distribution of injected 14C-oestrone and 14C-diethylstilbestrol: whole-animal autoradiography and impulse counting. Acta Endocrinol., 43, 587–591.

    PubMed  CAS  Google Scholar 

  • BERGMAN, K. (1978), Application of whole-body autoradiography to distribution studies of organic solvents, In: Proc. Int. Symp. on the Control of Air Pollution in the Working Environment, Part II Solvents, Welding, pp. 128–139. International Labour Office, Geneva.

    Google Scholar 

  • BERGMAN, K. (1979), Whole-body autoradiography and allied tracer techniques in distribution and elimination studies of some organic solvents. Scand. J. Work Environ. Health, 5, (Suppl. 1), 1–163.

    Article  PubMed  Google Scholar 

  • BERGMAN, K. (1983), Application and results of whole-body autoradiography in distribution studies of organic solvents. C.R.C. Crit. Rev. Toxicol., 12, 59–118.

    Article  CAS  Google Scholar 

  • BERGMAN, K. (1986), Application of whole-body autoradiography to distribution studies of volatile substances. Uppsala J. Med. Sci., 91, 269–272.

    Article  CAS  Google Scholar 

  • BERGMAN, G. and HAMMARSTRĂ–M, L. (1969), Research on calcified dental tissues. The value of combining different methods. Sven. TandlaeK. Tidskr., 62, 449–463.

    CAS  Google Scholar 

  • BERGMAN, K. and TJĂ„LVE, H. (1977), Three step autoradiography of organic solvents and plastic monomers to register total radioactivity, non volatile metabolites and non-extractable metabolites. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 22–23.

    Google Scholar 

  • BERLIN, M. and ULLBERG, S. (1963), Accumulation and retention of mercury in the mouse. Arch. Environ. Health, 6, 589–601.

    PubMed  CAS  Google Scholar 

  • BONNER, W.M. and LASKEY, R.A. (1974), A film detection method for tritiumlabelled proteins and nucleic acids in Polyacrylamide gels. Eur. J. Biochem., 46, 83–89.

    Article  PubMed  CAS  Google Scholar 

  • BLASBERG, R.G., GAZENDAM, J., PATLAK, C.S. and FENSTERMACHER, J.D. (1980), Quantitative autoradiographic studies of brain edema and a comparaison of multi-isotope autoradiographic techniques. In: Advances in Neurology, Vol. 28, (Cervos-Navarro, J. and Ferzi, R., eds.), pp. 255–261. Raven Press, New York.

    Google Scholar 

  • BLOMQUIST, L. (1972), Mode of accumulation of iodophenylalanines in the exocrine pancreas and certain tumours. Acta Radiol., Suppl. 322, 1–140.

    Google Scholar 

  • BLOMQUIST, L. and HANNGREN, A. (1966), Fluorescence technique applied to whole-body sections for distribution studies of tetracyclines. Biochem. Pharmacol., 15, 215–219.

    Article  PubMed  CAS  Google Scholar 

  • BLOMQUIST, L. and HANNGREN, A. (1968), Whole-body autoradiography and fluorography of two tetracycline compounds in tumor bearing mice. Acta Med. Scand., 184, 1–11.

    Article  PubMed  CAS  Google Scholar 

  • BRITTEBO, E.B. (1982), Metabolism of progesterone by the nasal mucosa in mice and rats. Acta Pharmacol. Toxicol., 51, 441–445.

    Article  CAS  Google Scholar 

  • BRITTEBO, E.B., LINDGREN, A. and TJĂ„LVE, H. (1981a), Foetal distribution and metabolism of N-nitrosodiethylamine in mice. Acta Pharmacol. Toxicol., 48, 355–363.

    Article  CAS  Google Scholar 

  • BRITTEBO, E.B., LĂ–FBERG, B. and TJĂ„LVE, H. (1981b), Sites of metabolism of N-nitrosopyrrolidine in mice and rats. Xenobiotica, 11, 619–625.

    Article  PubMed  CAS  Google Scholar 

  • BRITTEBO, E.B., LĂ–FBERG, B. and TJĂ„LVE, H. (1981c), Sites of metabolism of N-nitrosodiethylamine in mice. Chem. Biol. Interact., 34, 209–221.

    Article  PubMed  CAS  Google Scholar 

  • COE, R.A.J. (1982), An evaluation of X-ray films suitable for autoradiography using C-beta-radiation. Int. J. Appl. Radiat. Isot., 35, 575–582.

    Article  Google Scholar 

  • COE, R.A.J., MAC DONALD, C.M. and INGS, R.M.J. (1986), The deposition of Isoxepac and several of its analogues in rat liver. Proc. Roy. Microsc. Soc., 21, 277. (Abstract).

    Google Scholar 

  • COHEN, E.N. and HOOD, N. (1969a), Application of low-temperature autoradiography to studies of the uptake and metabolism of volatile anesthetics in the mouse. I. Chloroform. Anesthesiology, 30, 306–314.

    Article  PubMed  CAS  Google Scholar 

  • COHEN, E.N. and HOOD, N. (1969b), Application of low-temperature autoradiography to studies of the uptake and metabolism of volatile anesthetics in the mouse. III. Halothane. Anesthesiology, 31, 553–559.

    Article  PubMed  CAS  Google Scholar 

  • COHEN, E.N., CHOW, K.L. and MATHERS, L. (1972), Autoradiographic distribution of volatile anesthetics within the brain. Anesthesiology, 37, 324–331.

    Article  PubMed  CAS  Google Scholar 

  • COHEN. Y. (1961), Le passage transplacentaire des mĂ©dicaments. ThĂ©rapie, 16, 509–520.

    CAS  Google Scholar 

  • COHEN, Y. and WEPIERRE, J.P. (1961), Methode d’étude autobiographique de substances marquĂ©es volatiles. Rapport Commissariat a l’Energie Atomique Saclay (France), No. 2071.

    Google Scholar 

  • CROSS, S.A.M. (1977), A quantitative method for whole-body autoradiography. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 32–33.

    Google Scholar 

  • CROSS, S.A.M., GROVES, A.D. and HESSELBO, T. (1974), A quantitative method for measuring radioactivity in tissues sectioned for whole-body autoradiography. Int. J. Appl. Radiat. Isot., 25, 381–386.

    Article  PubMed  CAS  Google Scholar 

  • CURTIS, C.G., CROSS, S.A.M., MC CULLOCH, R.J. and POWELL, G.M. (1981), Whole-body Autoradiography. Academic Press, London.

    Google Scholar 

  • D’ARGY, R. (1977), Tissue pieces from whole body sections used for chromatographic separation of metabolites. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 16–17.

    Google Scholar 

  • D’ARGY, R.(1986), Image analysis in whole body autoradiography. Uppsala J. Med. Sci., 91, 257–262.

    Article  Google Scholar 

  • D’ARGY, R. (1988), Short-lived radionuclides and image analysis in whole-body autoradiography: studies run in parallel with positron emission tomography. Acta University Uppsala, Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy, 40, 47pp.

    Google Scholar 

  • DEAK, S.T., CSAKY, K.G. and WADDELL, W.J. (1976), Localization and histochemical correlation of As by whole-body autoradiography in mice. J. Toxicol. Environ. Health, 1, 981–984.

    Article  PubMed  CAS  Google Scholar 

  • DENCKER, L. (1976), Tissue localization of some teratogens at early and late gestation related to fetal effects. Acta Pharm. Toxicol., 39, (Suppl. 1), 1–131.

    CAS  Google Scholar 

  • DENCKER, L. and ULLBERG, S. (1973), Uptake and distribution of drugs in the fetus. In: Proc. European Teratology Society, (Klike, M.E., Rychter, Z., Jelinek, R. and Kucera, J., eds.), Part 1, pp. 73–76. Acta Universitatis Carolinae Medica, Praha.

    Google Scholar 

  • DURAND S., BÉNARD, P. and BRAUN, J.P. (1980), Ethionine and methionine distribution and toxicity in the rat kidney. Toxicol. Lett., 5, 257–261.

    Article  PubMed  CAS  Google Scholar 

  • DWYER, B.E., DONATONI, P. and WASTERLAIN, C.G. (1982), A quantitative autoradiographic method for the measurement of local rates of brain protein synthesis. Neurochem. Res., 7, 563–572.

    Article  PubMed  CAS  Google Scholar 

  • FAND, I., SHARKEY, R.M., MC NALLY, W.P., BRILL, A.B., SOM, P., YAMAMOTO, K., PRIMUS, F.J. and GOLDENBERG, D.M. (1986), Quantitative whole-body autoradiography of radiolabeled antibody distribution in a xenografed human cancer model. Cancer Res., 46, 271–277.

    PubMed  CAS  Google Scholar 

  • FAREBROTHER, D.A. and CREASEY, D.M. (1976), Whole-body autoradiography. A β-radioluminescence method of detecting tritium. J. Microscopy, 108, 195–201.

    Article  CAS  Google Scholar 

  • FAREBROTHER, D.E. and WOODS, N.C. (1973), Permanent preparations of stained whole-body sections using “Tricolac”. J. Microsc., 97, 373–375.

    Article  PubMed  CAS  Google Scholar 

  • FEINGOLD, E., SESHADRI, S.B. and TRETIAK, O.J. (1986), Hardware and software design considerations in engineering an image processing workstation. Exp. Biol. Med., 11, 175–203.

    Google Scholar 

  • FILTHUTH, H. (1989), Digital autoradiography. Anew detector for radiochromatography and electrophoresis. In: Synthesis and Applications of Isotopically Labelled Compounds. (Baillie T.A. and Jones J.R., eds.), pp. 315–321. Elsevier, Amsterdam.

    Google Scholar 

  • FRANKLIN, E.R. (1980), The response of selected X ray films to 14C-beta radiation. Int. J. Appl. Rad. Isot., 31, 256–258.

    Article  Google Scholar 

  • FRANKLIN, E.R. (1983), Factors affecting the sensitivity of X-ray films used for whole-body autoradiograghy. Xenobiotica, 13, 163–169.

    Article  PubMed  CAS  Google Scholar 

  • FRANKLIN, E.R. and ROSS, D.A. (1988), Whole-body autoradiography In: The Design of Disposition and Metabolism Studies on Novel Compounds. (H.P.A. Illing ed.), pp. 63–82. C.R.C. Press, Boca Raton.

    Google Scholar 

  • FRANKLIN, E.R. and TAYLOR, D.C. (1981), Whole-body autoradiography (WB A) in toxicity testing. In: Testing for Toxicity, (Gorrod, J.W., ed.), pp. 45–60. Taylor and Francis, London.

    Google Scholar 

  • GILLBERG, P.G. JOSS AN, S.S. ASKMAR, H. and AQUILONIUS, S.M. (1986), Large-sections cryomicrotomy for in vitro receptor autoradiography. J. Pharmacol. Methods, 15, 169–180.

    Article  PubMed  CAS  Google Scholar 

  • GOOCHEE, C., RASBAND, W. and SOKOLOFF, L. (1980), Computerized densitometry and color coding of (14C)deoxyglucose autoradiographs. Ann. Neurol., 7, 359–364.

    Article  PubMed  CAS  Google Scholar 

  • HAMMARSTRĂ–M, L. (1968), Tetracycline in developing rat enamel in relation to pretein synthesis and maturation. Acta Odontol. Scand., 26, 337–356.

    Article  PubMed  Google Scholar 

  • HAMMARSTRĂ–M, L. (1986), Autoradiography and histochemistry of mineralized tissues by means of the Ullberg freeze-sectioning technique. Uppsala J. Med. Sci., 91, 239–243.

    Article  Google Scholar 

  • HAMMARSTRĂ–M, L.F. (1977), Histochemistry and autoradiography of hard tissues. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 46–47.

    Google Scholar 

  • HERZ, R.H. (1964), The Photographic Action of Ionizing Radiations. Wiley-Interscience, New-York.

    Google Scholar 

  • INGEBRITSEN, K. and NAFSTAD, I. (1983), Distribution and elimination of 14C-hexachlorobenzene atter single oral exposure in the male rat. Acta Pharmacol. Toxicol., 52, 254–260.

    Article  Google Scholar 

  • INGS, R.M.J. (1984), The mechanism binding of drugs and its implication. Drug Metab.Rev., 15, 1183–1212.

    Article  PubMed  CAS  Google Scholar 

  • IRONS, R.D. and CROSS, E.A. (1981), Standardization and calibration of whole-body autoradiography for routine semi-quantitative analysis of the distribution of 14C-labelled compounds in animal tissues. Toxicol. Appl. Pharmacol., 59, 250–256.

    Article  PubMed  CAS  Google Scholar 

  • JOHANSSON, E.B. and TJĂ„LVE, H. (1979a), Studies on the distribution and metabolism of N-(14C)nitrosopyrrolidine in mice. Chem. Biol. Interact., 26, 243–253.

    Article  Google Scholar 

  • JOHANSSON, E.B. and TJĂ„LVE, H. (1979b), Studies on the distribution and metabolism of 14C-dimethylnitrosamine in foetal and young mice. Acta Pharmacol. Toxicol., 45, 73–80.

    Article  Google Scholar 

  • KALBERER, F. (1966), A new method of macro-autoradiography. Atomlight, 51, 1–7.

    Google Scholar 

  • KAWAMOTO, T. and SHIMIZY, M. (1986), A method for preparing whole-body sections suitable for autoradiographic, histological and histochemical studies. Stain Technol., 61, 169–183.

    PubMed  CAS  Google Scholar 

  • KHAN, R.A., HUGHES, S., LAVENDER, P., LEON, M. and SPYROU, N. (1979), Autoradiography of technetium-labelled diphosphonate in rat bone. J. Bone Jt. Surg., 61, 221–224.

    Google Scholar 

  • LARSSON, B. (1979), Mechanisms of Accumulation of Foreign Substances in Melanin. Binding to Preformed Melanin and Incorporation into Growing Melanin. Ph. D. thesis, University of Uppsala, Uppsala, Sweden.

    Google Scholar 

  • LARSSON, B. and ULLBERG, S. (1977), A film for rapid registration of tritium. Science Tools, the LKB Instrument Journal, special issue on whole-body autoradiography, 30–32.

    Google Scholar 

  • LARSSON, B. and ULLBERG, S. (1977). Science Tools, The LKB Instrument Journal, special issue on whole-body autoradiography, 30–32.

    Google Scholar 

  • LARSSON, B. and ULLBERG, S. (1981), Whole-body autoradiography. J. Histochem. Cytochem., 29, 216–225.

    Article  PubMed  CAS  Google Scholar 

  • LARSSON, B., NILSSON, M. and TJĂ„LVE, H. (1981), The tissue disposition of gentamicin in rats. Acta Pharmacol., Toxicol., 48, 269–278.

    Article  CAS  Google Scholar 

  • LAURENT, F., BÉNARD P., CANAL, P. and SOULA, G. (1988), Autoradiographic distribution of (14C)-labelled pimonidazole in rhabdomyosarcona-bearing rats and pigmented mice. Cancer Chemother. Pharmacol., 22, 308–315.

    Article  PubMed  CAS  Google Scholar 

  • LAURENT, F., CANAL, P. and SOULA, G. (1989), Pharmacokinetics of Ro 03–8799 in mice bearing melanosarcoma: comparison with tumors without melanin. Int. J. Radiation Oncology Biol. Phys., 16, 1101–1103.

    Article  CAS  Google Scholar 

  • L’HERMITTE, M., DENAYER, P., GOLSTEIN, J., VIRSORO, E., VANHAELST, L., COPINSCHI, G. and ROB YN, C. (1978), Acute endocrine profile of sulpiride in the human. Clin. Endocrinol., 9, 195–204.

    Article  Google Scholar 

  • LINDQUIST, N.G. (1972), Autoradiographic-histochemical demonstration of tyrosinase activity in melanin-forming tissues of mammals. Acta Physiol. Scand., 92, 530–536.

    Article  Google Scholar 

  • LINDQUIST, N.G. (1973), Accumulation of drugs on melanin. Acta Radiol., Suppl. 325, 1–92.

    CAS  Google Scholar 

  • LINDQUIST, N.G. and ULLBERG, S. (1972), The melanin affinity of chloroquine and chlorpromazine studied by whole-body autoradiography. Acta Pharmacol. Toxicol., 31, (Suppl. 2), 1–32.

    Google Scholar 

  • LONGSHAW, S. and FOWLER, J.S.L. (1977), The development of a permanent, robust, stable, standard radioactive source for use in whole-body autoradiography. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 52–53.

    Google Scholar 

  • LONGSHAW, S. and FOWLER, J.S.L. (1978), A poly(methyl-14C)methacrylate source for use in whole-body autoradiography and beta-radiography. Xenobiotica, 8, 289–295.

    Article  PubMed  CAS  Google Scholar 

  • MASON, C.C. (1977), Ocular accumulation and toxicity of certain systematically administered drugs. J. Toxicol. Environ. Health, 2, 977–995.

    Article  PubMed  CAS  Google Scholar 

  • MASSAT, F. (1984), Contribution Ă  l’étude quantitative d’autoradiogrammes d’animaux entiers par microdensitomĂ©trie. Thèse Doct. Ing. C.N.A.M., Toulouse, France.

    Google Scholar 

  • MASUOKA, D.T. and PFEIFFER, E. (1977), An in vitro brain autoradiographic technique and a digital micro-densitometer for the quantitation of autoradio-grams. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 54–55.

    Google Scholar 

  • MCNALLY, W.P., ROTH, M., CHANG, T., UHLENDORF, P. and KRAUSE, B.R. (1988), Quantitative whole-body autoradiographic analysis of the tissue distribution of orally administered (14C)-cholesterol in hyper cholesterolemic rats. Atherosclerosis, 72, 221–229.

    Article  PubMed  CAS  Google Scholar 

  • MEES, C.E.K. (1971), The Theory of the Photographic Process. MacMillan, New-York.

    Google Scholar 

  • MORGAN, K.T. and STROLIN-BENEDETTI, M. (1977), Localisation of Tiapride and another substituted-benzamide in the pituitary gland of the rat. Acta Pharmacol. Toxicol., 41, Suppl. 1, 130–131.

    Google Scholar 

  • NAGATSUKA, S., HANAWA, S., HONDA, T. and HASEGAWA, M. (1988), Quantitative analysis of whole-body autoradiogram using a computer-assisted image analyzer. Xenobiotic Metab. Disp., 3, 121–135.

    Google Scholar 

  • NAGATSUKA, S., HANAWA, S., HONDA, T. and HASEGAWA, M. (1989), Quantitative whole-body autoradiography and its application. In: Synthesis and Applications of Isotopically-Labelled Compounds. (Baillie, T.A and Jones, J.R., eds.), pp. 493–498. Elsevier, Amsterdam.

    Google Scholar 

  • OSKARSSON, A. and TJĂ„LVE, H. (1979), An autoradiographic study on the distribution of 63NiCl2 in mice. Ann. Clin. Lab. Sci., 9, 47–59.

    PubMed  CAS  Google Scholar 

  • PALACIOS, J.M. (1984), Receptor autoradiography: the last ten years. J. Recept. Res., 4, 633–644.

    PubMed  CAS  Google Scholar 

  • PALACIOS, J.M., NIEHOFF, D.L. and KUHAR, M.J. (1981), Receptor autoradiography with tritium sensitive film: potential for computerized densitometry. Neurosci. Lett., 25, 101–105.

    Article  PubMed  CAS  Google Scholar 

  • PALACIOS, J.M., PROBST, A. and CORTES, R. (1986), Mapping receptors in the human brain. Trends Neurosci., 9, 284–289.

    Article  CAS  Google Scholar 

  • PELLERIN, P. (1957), Technique d’autoradiographie Ă  très basse tempĂ©rature. C.R. Acad. Sci., 244, 1555–1558.

    CAS  Google Scholar 

  • POTTS, A.M. (1962), The concentration of phenothiazines in the eye of experimental animals. Invest. Ophtalmol., 1, 522–530.

    CAS  Google Scholar 

  • PROBST, A., CORTES, R. and PALACIOS, J.M. (1985), Distribution of α2-adrenergic receptors in the human brainstem: an autoradiographic study using (3H)p-aminoclonidine. Eur. J. Pharmacol., 106, 477–488.

    Article  Google Scholar 

  • REY-CENEVAZ. Y. and SIMONNET, G. (1988), A simple and inexpensive means of intensifying 32P autoradiography Appl. Radiat. Isot., 39, 1093–1095.

    Article  CAS  Google Scholar 

  • RHODES, C. and LONGSHAW, S. (1977), Autoradiographic distribution study of a short acting anaesthetic. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 132–133.

    Google Scholar 

  • RICO, A.G., BÉNARD, P., BRAUN, J.P. and BURGAT-SACAZE, V. (1977), Cartoautoradiography: an application of macroscopic autoradiography to large species in veterinary pharmacokinetics. Acta Pharmacol. Toxicol., 41, Suppl. 1, 68–69.

    Google Scholar 

  • RICO, A.G., BÉNARD, P., BRAUN, J.P. and BURGAT-SACAZE, V. (1980), Whole-body autoradiography in metabolic studies of drugs and toxicants. In: Advances in Veterinary Science and Comparative Medicine, Vol. 24, (Cornelius, C.E. and Brandly, C.A., eds.), pp. 291–311. Academic Press, New-York.

    Google Scholar 

  • ROGERS, A.W. (1979), Techniques of autoradiography. Elsevier Scientific Publishing, Amsterdam.

    Google Scholar 

  • ROHLIN, M., LARSSON, A. and HAMMARSTRĂ–M, L. (1977), Distribution of mTc-labelled phosphorus compounds, 45Ca and 85Sr in diphosphonate-treated rats. Acta radiol., 16, 513–524.

    Article  CAS  Google Scholar 

  • SCHWEITZER, A. (1988a), Evaluation of Hyperfilm-betamax. Amersham Research News, 1, 12–13.

    Google Scholar 

  • SCHWEITZER, A. (1988b), Automatic processing of Hyperfilm-betamax for autoradiography. Amersham Research News, 1, 14–16.

    Google Scholar 

  • SCHWEITZER, A., FAHR, A. and NIEDERBERGER, W. (1987), A simple method for the quantitation of (14C) whole-body autoradiographs. Int. J. Appl. Rad. Isot., 38, 329–333.

    CAS  Google Scholar 

  • SHIMONO, R., WATANABE, M. and GOTO, H. (1982), A new method of preparation of whole-body sections for autoradiography and staining. Acta Anat. Nippon, 57, 15–21.

    PubMed  CAS  Google Scholar 

  • SHINDO, H. (1972), Applications of whole-body autoradiography to the studies of drug metabolism and disposition. Ann. Sankyo Res. Lab., 24, 1–72.

    CAS  Google Scholar 

  • SHINDO, H., NAKAJIMA, E. and SHIGEHARA, E. (1976), Autoradiographic studies on the distribution of quaternary ammonium compounds. IV Adsorption of bisonium ions to rat tissues as relealed by in vitro whole-body adsorption autoradiography. Chem. Pharm. Bull., 24, 2327–2334.

    Article  PubMed  CAS  Google Scholar 

  • SJĂ–STRAND, S.E., CASS ANO, G.B. and HANSSON, E. (1965), The distribution of 35S-chlorpromazine in mice studied by whole-body autoradiography. Arch. Int. Pharmacodyn. Ther., 156, 34–47.

    PubMed  Google Scholar 

  • SOINI, E. and KOJOLA, H. (1977), An automatic gas counter for quantitative microdetermination of tritium in biological material. Acta Pharmacol. Toxicol, 41, (Suppl.1), 78–79.

    Google Scholar 

  • STEFAN, Y. and BENAKIS, A. (1976), Etude ultrastructurale des stimulations exercĂ©es par le Sulpiride sur les cellules Ă  prolactine et les cellules gonadotropes de l’hypophyse du rat mâle et femelle. Ann. Endocr. (Paris), 37, 375–388.

    CAS  Google Scholar 

  • STEINBACH-LEBBIN, C., WASER, P.G. and CHANG-SIN-REN, A. (1978), Radioactive determination: quantitative correlation of whole-body autoradiography as compared to oxygen combustion of tissues probes. Eur. J. Drug Metab. Pharmacokin., 3, 155–159.

    Article  CAS  Google Scholar 

  • TAMMELA, M. (1985), Tissue localization of some organic and inorganic cations: binding to melanin and cartilage. Ph.D. Thesis, University of Uppsala, Uppsala, Sweden.

    Google Scholar 

  • TJĂ„LVE, H. (1971), Catechol and indolamines in some encodrine cell systems. An autoradiographical, histochemical and radioimmunological study. Acta Physiol. Scand., Suppl. 360, 52–86.

    Google Scholar 

  • UKITA, T. (1971), Experimental follow-up study of mercury intoxications. I. Behaviour of mercury compounds in body of mammalians. Kagaku, 41, 557–568.

    CAS  Google Scholar 

  • ULLBERG, S. (1954), Studies on the distribution and fate of S-labeled benzyl-penicillin in the body. Acta Radiol., Suppl. 118, 1–110.

    CAS  Google Scholar 

  • ULLBERG, S. (1958), Autoradiographic studies on the distribution of labelled drugs in the body. In: Proceedings of the Second U.N. International Conference on the Peaceful Uses of Atomic Energy, pp. 248–254. Pergamon Press, New-York.

    Google Scholar 

  • ULLBERG, S. (1963), Autoradiographic localization in the tissues of drugs and metabolites. In: Proceedings First International Pharmacology Conference, (Lowry, O.E., ed.), Volume 5, pp. 29–36. Pergamon Press, Oxford.

    Google Scholar 

  • ULLBERG, S. (1965), Specific localization of labelled hormones and vitamins: whole-body autoradiographic observations. In: Isotopes in Experimental Pharmacology, (Roth, L.J., ed.), pp. 63–74. University of Chicago Press, Chicago.

    Google Scholar 

  • ULLBERG, S. (1969), Distribution and fate of drugs used in feeds. In: The Use of Drugs in Animal Feeds, pp. 225–243.National Academy of Sciences, Washington D.C.

    Google Scholar 

  • ULLBERG, S. (1973), Autoradiography in fetal pharmacology. In: Fetal Pharmacology, (Boreus, L.O., ed.), pp. 55–73. Raven Press, New-York.

    Google Scholar 

  • ULLBERG, S. (1977), The technique of whole-body autoradiography. Cryosectioning of large specimens, Science Tools, the LKB Instrument Journal, special issue on whole-body autoradiography, 1–29.

    Google Scholar 

  • ULLBERG, S. and APPELGREN, L.E. (1969), Experiences in locating drugs at different levels of resolution. In: Autoradiography of Diffusible Substances, (Roth, L.J. and Stumpf, W., eds.), pp. 279–299. Academic Press, New-York.

    Google Scholar 

  • ULLBERG, S. and D’ARGY, R. (1977), Conditions for in vitro autoradiography using 125I-gonadotrophins (LH and HGG) as test substances. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 84–85.

    Google Scholar 

  • ULLBERG, S. and HAMMARSTRĂ–M, L. (1969), Localization in relation to action. In: International Conference on Radioactive Isotopes in Pharmacology, (Waser, P.G. and Glasson, B., eds.), pp. 225–241. Interscience, Chichester.

    Google Scholar 

  • ULLBERG, S. and LARSSON, B. (1981), Whole-body autoradiography. In: Methods in Enzymology, Vol. 77, (Jakoby, W.B., ed.), pp. 64–80. Academic Press, New-York.

    Google Scholar 

  • ULLBERG, S., DENCKER, L. and DANIELSSON, B.R.G. (1982), The distribution of drugs and other agents in the fetus. In: Developmental Toxicology, (Snell, K., ed.), pp. 125–163. Croom Helm, London.

    Google Scholar 

  • ULLBERG, S., HAMMARSTRĂ–M, L. and APPELGREN, L.E. (1972), Autoradiography in pharmacology. In: International Encyclopedia of Pharmacology, Section 78, Vol. 1, (Cohen, Y., ed.), Chap. 7., pp. 221–239. Pergamon Press, Oxford.

    Google Scholar 

  • VALETTE, G., COHEN, Y. and WEPIERRE, J. (1962), RĂ©sorption pulmonaire et localisation chez la souris du p-cymène marquĂ© au carbone-14. C.R. Acad. Sci., 255, 596–598.

    CAS  Google Scholar 

  • WADDELL, W.J. (1972), Localization and metabolism of drugs in the fetus. Fed. Proc., 31, 52–61.

    PubMed  CAS  Google Scholar 

  • WADDELL, W.J. and MARLOWE, G.C. (1976), Disposition of drugs in the fetus. In: Perinatal Pharmacology and Therapeutics, (Mirkin, B.L., ed.), pp. 119–268. Academic Press, New-York.

    Google Scholar 

  • WADDELL, W.J. and MARLOWE, G.C. (1981), Biochemical regulation of the accessibility of teratogens to the developing embryo. In: The Biochemical Basis of Teratogenesis, (Juchau, M.R., ed.), Chap. 1., pp. 1–62. Elsevier/North-Holland, Amsterdam.

    Google Scholar 

  • WATANABE, M., GOTO, H., MATSUSHIMA, M., SHIMONO, R. and KIHARA, T. (1983), Distribution of glucose-6-phosphatase activity in mice studied by in vitro whole-body autoradiography. J. Histochem. Cytochem., 31, 1426–1437.

    Article  PubMed  CAS  Google Scholar 

  • WATANABE, M. and KIHARA, T. (1978), Preparation and stain of whole-body sections. Cell. Mol. Biol., 23, 311–315.

    CAS  Google Scholar 

  • WATANABE, M. and SHIMADA, M. (1975), Staining method for whole-body autoradiography. Stain Technol., 50, 239–243.

    PubMed  CAS  Google Scholar 

  • WATANABE, M., SHIMADA, M., KURIMOTI, K., MARUYAMA, Y. and IJICHI, H. (1975), Section staining technique for histological analysis of whole-body autoradiographs. Acta Anat. Nippon, 50, 250–261.

    PubMed  CAS  Google Scholar 

  • WEBER, H., WINKELMANN, H., WEGNER, L.A. and PATZSCHKE, K. (1977), The determination of carbon-14 in whole-body sections by combustion and liquid scintillation counting. Acta Pharmacol. Toxicol., 41, (Suppl. 1), 90–91.

    Google Scholar 

  • WEGNER, L.A. and PATZSCHKE, K. (1976), Zur Bstimmung von 14C and 3H in ganskorper Schnitten. Int. J. Appl. Radiat. Isot., 27, 573–583.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. Patrick BĂ©nard
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Peter Bach and John Baker

About this chapter

Cite this chapter

BĂ©nard, P. (1991). Whole-Body Autoradiography of Pharmaceuticals and Chemicals. In: Histochemical and Immunohistochemical Techniques. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3094-3_2

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-3094-3_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5370-9

  • Online ISBN: 978-94-011-3094-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation