Summary
Environmental pollutants account for 1–3% of the extractable, organically bound chlorine (EOCl) found in bivalve lipids. In this work, bivalve lipids were converted to fatty acid methyl esters (FAMEs). The EOCl in the FAMEs and in acidic compounds was examined after liquid-liquid extraction using neutron activation analysis for chlorine determination. Gas chromatography with halogen selective, electrolytic conductivity detection (GC/ELCD) was employed to study the occurrence of halogenated FAMEs. Following esterification, most of the EOCl was recovered in the FAME-containing fraction. However, silica gel chromatography showed that more than 50% of the EOCl consisted of compounds of higher polarity than the chlorinated FAMEs normally found in fish extracts. By using GC/ELCD, up to 25% of the EOCl was detected as halogenated fatty acids. A complex pattern of halogenated fatty acids was found in bivalves from the Baltic Sea. This pattern was simplified to one consisting of only a few halogenated fatty acids in bivalves from West Scandinavian waters. These acids seem to persist in the ecosystem. It is possible that up to 40% of the EOCl remained in a brown, organic material of polar character that was coextracted with the FAMEs.
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Wesén, C., Mu, H., Sundin, P., Ringstad, O., Odham, G. (1995). Occurrence of halogenated fatty acids in bivalve lipids. In: Grimvall, A., de Leer, E.W.B. (eds) Naturally-Produced Organohalogens. Environment & Chemistry, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0061-8_28
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DOI: https://doi.org/10.1007/978-94-011-0061-8_28
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