Abstract
For an improved understanding of the correlation between the formation of chromosome aberrations induced e.g., by ionising radiation, and the three dimensional structure of the genome, computer simulations of the human cell nucleus were applied to calculate relative translocation rates (Kreth et al., 1998). In the present study an extended computer model was applied taking into account the observed different morphology of both X chromosomes in human female cell nuclei. In the modelling of nuclear genome structure, “tensegrity forces” were introduced which allowed the simulation of different compartmentalisations of a chromosome territory. With the simulation of the observed surface ratio Aenv(Xa)/Aenv(Xi) ~ 1.4 (Aenv -enveloping surface) between the two X chromosome territories, marked differences in the translocation rates of both territories were predicted. These computer simulations show that the morphology of a given chromosome territory may have a profound influence on its aberration frequency allowing to establish a quantitatively testable correlation between these observables.
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Bornfleth, H., Sätzler, K., Eils, R. and Cremer, C. (1998) High-precision distance measurements and volumeconserving segmentation of objects near and below the resolution limit in three dimensional confocal fluorescence microscopy. J. Microscopy 189(2): 118-136
Bornfleth, H., Edelmann, P., Zink, D., Cremer, T. and Cremer, C. (1999) Quantitative motion analysis of subchromosomal foci in living cells using four-dimensional microscopy. Biophys. J. 77: 2871-2886.
Cremer, T., Kurz, A., Zirbel, R., Dietzel, S., Rinke, B., Schrö ck, E., Speicher, M.R., Mathieu, U., Jauch A., Emmerich, P., Scherthan, H., Ried, T., Cremer, C. and Lichter, P. (1993) Role of chromosome territories in the functional compartmentalization of the cell nucleus. Cold Spring Harb. Symp. Quant. Biol. 58: 777-792
Cremer, T., Dietzel, S., Eils, R., Lichter, P. and Cremer C (1995) Chromosome territories, nuclear matrix filaments and interchromatin channels: A topological view on nuclear architecture and function. Kew Chromosome Conference IV (P.E. Brandham, and M.D. Benett, eds. Royal Botanic Gardens, Kew: 63-81)
Cremer, C., Münkel, Ch. Granzow, M., Jauch, A., Dietzel, S., Eils, R., Guan, X-Y., Meltzer, P.S., Trent, J,M., Langowski, J. and Cremer, T. (1996) Nuclear architecture and the induction of chromosomal aberrations. Mutation Research 366: 97-116.
Cremer, T., Kreth, G., Köster, H. , Fink, R.H.A., Heintzmann, R., Cremer, M., Solovei, I., Zink, D. and Cremer, C, (2000) Chromosome territories, interchromatin domain compartment and the nuclear matrix : an integrated view of the functional nuclear architecture, Critical Reviews in Eucaryotic Gene Expression, 12(2): 179-212.
Dietzel, S., Eils, R., Sätzler, K., Bomfleth, H., Jauch, A., Cremer, C. and Cremer, T. (1998) Evidence against a looped structure of the inactive human X-chromosome territory. Experimental Cell Research, 240: 187-196.
Edwards, A. A., Moiseenko, V.V. and Nlkjoo, H. (1994) Modelling of DNA breaks and the formation of chromosome aberrations. Radial Biol 66 5: 633-637.
Eils, R., Dietzel, S., Bertin, E., Schrdck, E., Speicher, M.R., Ried, T., Robert-Nicoud, M., Cremer, C. and Cremer, T. (1996) Three-dimensional reconstruction of painted human interphase chromosomes: active and inactive X chromosome territories have similar volumes, but differ in shape and surface structure. J. Cell Biol 135: 1427-1440.
Gundersen, H.J.G. and Jensen, Z.B. (1987) The efficiency of systematic sampling in stereology and its prediction. J. Microscopy 147: 229-263.
Herrmann, H., Munick, M.D., Brettel, M., Fouquet, B. and Markl, J. (1996) Vimentin in a cold-water fish, the rainbow trout: highly conserved primary structure but unique assembly properties. J. Cell Sci. 109: 569-578.
Houchmandzadeh, B., Marko, J.F., Chatenay, D. and Libchaber, A. (1997) Elasticity and structure of eukaryote chromosomes studied by micromanipulation and micropipette aspiration. J. Cell Biol. 139(1): 1-12.
Ingber, D.E. (1993) Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton. J. Cell Science 104: 613-627.
Knoch, T.A. (1998) Three-dimensional organization of chromosome territories in simulation and experiment. Diploma Thesis, University of Heidelberg.
Kreth, G., Münkel, Ch., Langowski, J., Cremer, T. and Cremer, C. (1998) Chromatin structure and chromatin aberrations: Modeling of damage induced by isotropic and localized irradiation. Mutation Research 404: 77-88.
Lamond, A.I. and Earnshaw, W.C. (1998) Structure and function in the nucleus. Science 280: 547-553.
Lucas, J.N., Awa, A., Straume, T., Poggensee, M., Kodama, Y., Nakano, M., Ohtaki, K., Weier, H., Pinkel, D., Gray, J. and Littlefield, G. (1992) Rapid translocation frequency analysis in humans decades after exposure to ionizing radiation. Int. J. Radial. Biol. 62: No. 1, 53-63.
Lucas, J.N. and Sachs, R.K. (1993) Using three-color chromosome painting to test chromosome aberration models. Proc. Natl. Acad. Sci. USA 90: 1484-1487
Lichter, P., Cremer, T., Borden, J., Manuelidis, L. and Ward, D.C. (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum. Genet. 80: 224.
Maniotis, A.J., Chen, C.S. and Ingber, D.E. (1997) Demonstration of mechanical connections between integrins, cytoskeletal filaments and nucleoplasm that stabilize nuclear structure. Proc. Natl. Acad. Sci. USA 4: 94(3): 849-854.
Munkel, Ch., and Langowski, J. (1998) Chromosome structure predicted by a polymer model. Phys. Rev. E 57: 5888-5896.
Münkel, Ch., Eils, R., Dietzel, S., Zink, D., Mehring, C., Wedemann, G., Cremer, T. and Langowski, J. (1999) Compartmentalization of interphase chromosomes observed in simulation and experiment. J. Mol. Biol. 285: 1053-1065.
Mühlmann-Diaz, M.C. and Bedford, J.S. (1994) A comparison of radiation-induced aberrations in human cells involving early and late replicating X-chromosomes. In: G. Obe and A.T. Natarajan (Eds.), Chromosomal Alterations, Origin and Significance. Springer-Verlag, Berlin. pp. 125-131.
Pinkel, D., Landegent, J., Collins, C, Fuscoe, J., Segarves, R., Lucas, J. and Gray J.W. (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4. Proc. Natl. Acad. Sci. 85: 9138.
Rinke, B., Bischoff, A., Meffert, M.C., Scharschmidt, R., Hausmann, M., Stelzer, E.H.K., Cremer, T. and Cremer, C. (1995) Volume ratios of painted chromosome territories 5, 7 and X in female human cell nuclei studied with confocal laser microscopy and the Cavalieri estimator. Bioimaging 3: 1-11.
Savage, J.R.K. and Papworth, D.G. (1973) The relationship of radiation-induced dicentric yield to chromosome arm number. Mutation Research 19: 139-143
Strasburger, E. (1905) Die stofflichen Grundlagen der Vererbung im organischen Reich. Gustav. Fischer, Jena, Germany.
Tanaka, K., Kamada, N., Ohkita, T. and Kuramoto, A. (1983) Non-random distribution of chromosome breaks in lymphocytes of atomic bomb survivors. Journal Radiation Research (Japan) 24: 291-304.
Walker, C.L., Cargile, C.B., Floy, K.M., Delannoy, M. and Migeon, B.R. (1991) The Barr body is a looped X chromosome formed by telomere association. Proc. Natl. Acad. Sci. USA 88: 6191-6195.
Zink, D. and Cremer, T. (1998) Chromosome dynamics in nuclei of living cells. Curr. Biol. 8: R321-324.
Zink, D., Bornfleth, H., Visser, A., Cremer, C. and Cremer, T. (1999) Organization of early and late replicating DNA in human chromosome territories. Exp. Cell Res. 247(1): 176-188.
Zirbel, R.M., Mathieu, U., Kurz, A., Cremer, T., Lichter, P. (1993) Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries. Chromosome Res. 1: 92-106.
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© 2002 Narosa Publishing House, New Delhi, India
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Kreth, G., Edelmann, P., Münkel, C., Langowski, J., Cremer, C. (2002). Translocation Frequencies for X and Y Chromosomes Predicted by Computer Simulations of Nuclear Structure. In: Sobit, R.C., Obe, G., Athwal, R.S. (eds) Some Aspects of Chromosome Structure and Functions. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0334-6_7
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DOI: https://doi.org/10.1007/978-94-010-0334-6_7
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