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Cytogenetic and Molecular Analysis of Heterochromatic Areas in the Holocentric Chromosomes of Different Aphid Species

  • Gian Carlo Manicardi
  • Mauro Mandrioli
  • Davide Bizzaro
  • Umberto Bianchi

Abstract

In this paper we review recent work concerning both cytogenetic and molecular features of constitutive heterochromatin in the holocentric chromosomes of different aphid species. Sections include an extensive survey regarding chromosomal localization and DNA base composition of Cpositive areas together with a cytological and molecular analysis of nucleolar organizer regions. The possible role of mitotic crossing over on genetic variability in parthenogenetic lineages is also briefly reviewed.

Key words

Aphid holocentric chromosomes heterochromatin satellite DNA NORs heteromorphism 

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References

  1. Arnheim, N., Krystal, M., Schmickel, R., Wilson, G., Ryder, O. and Zimmer, E. (1980) Molecular evidence for genetic exchange among ribosomal genes on non-homologous chromosomes in man and apes. Proc. Natl. Acad. Sci. USA 77: 7323-7327PubMedCrossRefGoogle Scholar
  2. Birky, C.W. (1996) Heterozygosity, heteromorphy and phylogenetic trees in asexual eukaryotes. Genetics 144: 427-437PubMedGoogle Scholar
  3. Bizzaro, D., Manicardi, G.C. and Bianchi, U. (1996) Chromosomal localization of a highly repeated EcoRI DNA fragment in Megoura viciae (Homoptera, Aphididae) by nick translation and FISH. Chromosome Res. 4: 392-396PubMedCrossRefGoogle Scholar
  4. Bizzaro, D., Mandrioli, M., Mul, E., Manicardi, G.C. and Bianchi, U. (1997) Cloning and sequencing of two DNA satellites in the aphid Megoura viciae. Atti. AGI, Vol. XLIII, pp. 17-18Google Scholar
  5. Bizzaro, D., Barbolini, E., Mandrioli, M., Mazzoni, E. and Manicardi, G.C. (1999) Cytogenetic characterization of the holocentric chromosomes in the aphids Myzus varians and Myzus cerasi. Caryologia, 52: 81-85Google Scholar
  6. Bizzaro, D., Barbolini, E., Mandrioli, M. and Manicardi, G.C. Molecular cytogenetics dissection of X-chromosomes restricted heterochromatin in the insect Aphis sambuci. Chromosome Res, submitted.Google Scholar
  7. Blackman, R.L. (1976) Cytogenetics of two species of Euceraphis (Homoptera, Aphididae). Chromosoma 56: 393-408PubMedCrossRefGoogle Scholar
  8. Blackman, R.L. (1978) Early development of the parthenogenetic egg in three species of aphids (Homoptera, Aphididae). Int. J. Insect. Morphol. Embryol.7: 33-44CrossRefGoogle Scholar
  9. Blackman, R.L. (1980) Chromosome numbers in the Aphididae and their taxonomic significance. Syst. Entomol. 5: 7-25CrossRefGoogle Scholar
  10. Blackman, R.L. (1987) Reproduction, cytogenetics and development, In: Minsk, A.K., Harrewijn P., eds. Aphids: their Biology, Natural Enemies and Control. Vol. A. Amsterdam: Elsevier, pp. 163-195Google Scholar
  11. Blackman, R.L. (1990) The chromosome of Lachnidae. Acta. Phytopathol Entomol Hung. 25: 273-282Google Scholar
  12. Blackman, R.L. and Hales, D.F. (1986) Behaviour of the X chromosomes during growth and maturation of parthenogenetic eggs of Amphorophora tuberculata (Homoptera, Aphididae) in relation to sex determination. Chromosoma 94: 59-64CrossRefGoogle Scholar
  13. Blackman, R.L. and Spence, J.M. (1996) Ribosomal DNA is frequently concentrated on only one X chromosome in permanently apomiptic aphids, but this does not inhibit male determination. Chromosome Res. 4: 314-320PubMedCrossRefGoogle Scholar
  14. Burkholder, G.D. (1989) Morphological and biochemical effects of endonucleases on isolated mammalian chromosomes in vitro. Chromosoma 97: 347-355PubMedCrossRefGoogle Scholar
  15. Camacho, J.P.M., Belda, J. and Cabrero, J. (1985) Meiotic behaviour of the holocentric chromosomes of Nezara viridula (Insecta, Heteroptera) analyzed by C-banding and silver impregnation. Can. J. Genet. Cytol. 27: 490-497Google Scholar
  16. Carvalho, G.R., MacLean, N., Wratten, S.D., Carter, R.E. and Thurston, J.P. (1991) Differentiation of aphid clones using DNA fingerprints from individual aphids. Proc. R. Soc. London B 243: 109-114CrossRefGoogle Scholar
  17. Collet, C. and Westerman, M. (1984) Interspersed distribution patterns of C-bands and satellite DNA in the holocentric chromosomes of Luzula flaccida (Juncaceae). Genetica 63: 175-179CrossRefGoogle Scholar
  18. De La Torre, J., Sumner, A.T., Gosalvez, J. and Stuppia, L. (1992) The distribution of genes on human chromosomes as studied by in situ nick translation. Genome. 35: 890-894PubMedCrossRefGoogle Scholar
  19. Dover, G.A. and Tautz, D. (1986) Conversion and divergence in multigene families: alternatives to selection and drift. Philos Trans. R. Soc. Lond. B 312: 275-289CrossRefGoogle Scholar
  20. Dover, G.A., Brown, S., Coen, E., Dallas, J., Strachan, T. and Trick, M. (1982) The dynamics of genome evolution and species differentiation. In: Dover, G.A., Flavell, R.B. eds., Genome evolution. Academic Press, pp. 343-372Google Scholar
  21. Fenton, B., Birch, A.N.E., Malloch, G., Woodford, J.A.T. and Gonzalez, C. (1994) Molecular analysis of ribosomal DNA from the aphid Amphorophora idaei and an associated fungal organism. Insect. Mol. Biol. 3: 183-189PubMedCrossRefGoogle Scholar
  22. Frankham, R., Briscoe, D.A. and Nurthen, R.K. (1978) Unequal crossing over at the rRNA locus as a source of quantitative genetic variation. Nature 272: 80-89PubMedCrossRefGoogle Scholar
  23. Galli, E. and Manicardi, G.C. (1998) Cytogenetic analysis on the gall generation of two aphid species: Tetraneura nigriabdominalis and Tetraneura ulmi. Caryologia. 51: 235-243Google Scholar
  24. Gautam, D.C., Crema, R. and Bonvicini Pagliai, A.M. (1993) Cytogenetic mechanisms in aphids. Boll. Zool. 60: 233-244Google Scholar
  25. Goday, C. and Pimpinelli, S. (1984) Chromosome organization and heterochromatin elimination in Parascaris. Science 224: 411-413PubMedCrossRefGoogle Scholar
  26. Gosalvez, J., Lopez-Fernandez, C., Ferrucci, L. and Mezzanotte, R. (1989) DNA base composition is not the only factor for restriction endonuclease activity on metaphase chromosomes: evidences using isoschizomers. Cytogenet Cell Genet. 50: 142-144PubMedCrossRefGoogle Scholar
  27. Hales, D.F. (1989) The chromosomes of Schoutedenia lutea (Homoptera, Aphididae, Greenideinae), with an account of meiosis in the male. Chromosoma 98: 295-300PubMedCrossRefGoogle Scholar
  28. Hales, D.F., Tomiuk, J., Wohrmann, K. and Sunnuks, P. (1997) Evolutionary and genetic aspects of aphid biology: A review. Eur. J. Entomol. 94: 1-55Google Scholar
  29. Herrero, P., de La Torre, J., Arano, B., Gosalvez, J. and Sumner, A.T. (1995) Patterns of DNase sensitivity in the chromosomes of Rana perezi (Amphibia: Anura). Genome. 38: 339-343PubMedCrossRefGoogle Scholar
  30. Huang, G.S. and Keil, R.L. (1995) Requirements for activity of the yeast mitotic recombination hotspot HOT1: RNA polymerase I and multiple cis-acting sequences. Genetics 141: 845-855PubMedGoogle Scholar
  31. John, B. (1988) The biology of heterochromatin. In: Verna, R.S. ed. Heterochromatin: molecular and structural aspects. Cambridge University Press, Cambridge, pp. 1-147Google Scholar
  32. John, B. and Miklos, G.L.G. (1979) Functional aspects of satellite DNA and heterochromatin. Int Rev. Cytol. 58: 1-114PubMedCrossRefGoogle Scholar
  33. Khuda-Bukhsh, A.R. and Datta, S. (1981) A preliminary note on the mitotic chromosome aberrations in embryos of whole-body X-irradiated parthenogenetic viviparous Aphis gossypii (Homoptera, Aphididae). Chrom Inform Serv. 30: 4-5Google Scholar
  34. Khuda-Bukhsh, A.R. and Kar. I (1990) Karyotypic studies in twenty-seven species of aphids (Homoptera, Aphididae), from India. Cytologia 55: 231-41Google Scholar
  35. Koo, H.S., Wu, H.M. and Crothers, D.M. (1986) DNA bending at adenine-thymine tracts. Nature 320: 501-504PubMedCrossRefGoogle Scholar
  36. Kuznetsova, V.G. and Sapunov, V.B. (1987) Effect of X-rays on the morphological and karyological inconstancy of aphids. In: Population Structure, Genetics and Taxonomy of Aphids and Thysanoptera. Proc. Int. Symp, Smolenice, Czechoslovakia, 1985, pp. 134-138Google Scholar
  37. Kuznetsova, V.G. and Maryanska-Nadachowska, A. (1993) C-heterochromatin and NOR's in X-chromosomes of aphid Dysaphis anthrisci Born. Karyosyst. Invert. Anim. 2: 16-18Google Scholar
  38. Kuznetsova, V.G., Maryanska-Nadachowska, A. and Nokkala, S. (1997) C-banded karyotype of psyllid species Aphalaria calthae (L) (Psylloidea, Homoptera, Insecta), Cytologia 62: 237-239Google Scholar
  39. Lagowsky, J.M., Yu. M-Y.W., Forrest, H.S. and Laird, CD. (1973) Dispersity of repeat DNA sequences in Oncopeltus fasciatus, an organism with diffuse centromeres. Chromosoma 43: 349-373CrossRefGoogle Scholar
  40. Lyckegaard, E.M. and Clark, A.G. (1991) Evolution of ribosomal RNA gene copy number on the sex chromosomes of Drosophila melanogaster. Mol. Biol. Evol. 8: 458-474PubMedGoogle Scholar
  41. Mandrioli, M., Bizzaro, D., Giusti, M., Manicardi, G.C. and Bianchi, U. (1999a) The role of rDNA genes during somatic X chromosomes association in the aphid Acyrthosiphon pisum. Genome. 7: 157-162Google Scholar
  42. Mandrioli, M., Bizzaro, D., Manicardi, G.C., Gionghi, D., Bassoli, L. and Bianchi, U. (1999b) Cytogenetic and molecular characterization of a highly repeated DNA sequence in the peach potato aphid Myzus persicae. Chromosoma, 108: 436-442PubMedCrossRefGoogle Scholar
  43. Mandrioli, M., Manicardi, G.C., Bizzaro, D. and Bianchi, U. (1999c) NOR heteromorphism within a parthenogenetic lineage of the aphid Megoura viciae. Chromosome Res. 7: 157-162PubMedCrossRefGoogle Scholar
  44. Mandrioli, M., Ganassi, S., Bizzaro, D. and Manicardi, G.C. (1999d) Cytogenetic analysis of the holocentric chromosomes of the aphid Schizaphis graminum. Hereditas.Google Scholar
  45. Manicardi, G.C. and Gautam, D.C. (1994) Cytogenetic investigation on the holocentric chromosomes of Tetraneurella akinire (Sasaki) (Homoptera, Pemphigidae). Caryologia 47: 159-165Google Scholar
  46. Manicardi, G.C., Gautam, D.C., Bizzaro, D., Guicciardi, E., Bonvicini Pagliai, A.M. and Bianchi, U. (1991) Chromosome banding in Aphids: G., C., AluI, and HaeIII banding patterns in Megoura viciae (Homoptera, Aphididae). Genome. 34: 661-665CrossRefGoogle Scholar
  47. Manicardi, G.C., Bizzaro, D., Aloisi, P., Bonvicini-Pagliai, A.M. and Bianchi, U. (1992) New approaches to the study of aphid chromosome. Proceeding of the Fourth ECE/XIII, SIEEC, .Godollo. 1992. Budapest: Hungarian Natural History Museum, pp. 85-88Google Scholar
  48. Manicardi, G.C., Bizzaro, D., Azzoni, P. and Bianchi, U. (1994) Cytological and electrophoretic analysis of DNA methylation in the holocentric chromosomes of Megoura viciae (Homoptera, Aphididae). Genome. 37: 625-630PubMedCrossRefGoogle Scholar
  49. Manicardi, G.C., Bizzaro, D., Galli, E. and Bianchi, U. (1996) Heterochromatin heterogeneity in the holocentric X chromatin of Megoura viciae (Homoptera, Aphididae). Genome. 39: 465-470PubMedCrossRefGoogle Scholar
  50. Manicardi, G.C., Mandrioli, M., Bizzaro, D. and Bianchi, U. (1998a) Silver staining as a new banding technique to identify aphid chromosomes. Chromosome Res. 6: 55-57PubMedCrossRefGoogle Scholar
  51. Manicardi, G.C., Mandrioli, M., Bizzaro, D. and Bianchi, U. (1998b) Patterns of DNase I sensitivity in the holocentric chromosomes of the aphid Megoura viciae. Genome 41: 169-172Google Scholar
  52. Manicardi, G,C., Mandrioli, M., Bizzaro, D., Bianchi, U. and Crema, R. (1998c) Occurrence of NORs heteromorphism in the two X chromosomes of different aphid species. In: Nieto Faria, J.M., Dixon, A.G. edsGoogle Scholar
  53. Aphids in Natural and Managed Ecosystems, Fifth International Symposium on Aphids, Leon (Spain) 1997, pp. 91-95Google Scholar
  54. Martinez-Balbas, A., Rodriguez-Campos, A., Garcia-Ramirez, M., Sainz, J., Carrera, P., Aymami, J. and Azorin, F. (1990) Satellite DNAs contain sequences that induce curvature. Biochemistry 29: 2342-2348.PubMedCrossRefGoogle Scholar
  55. Mezzanotte, R., Ferrucci, L., Vanni, R. and Sumner, A.T. (1985) Some factors affecting the action of restriction endonucleases on human metaphase chromosomes. Exp. Cell. Res. 161: 247-253.PubMedCrossRefGoogle Scholar
  56. Mezzanotte, R., Rossino, R., Nieddu, M., Lopez-Fernandez, C. and Gosalvez, J. (1992) The DNA fragments produced by AluI and BstNI digestion of fixed mouse chromosomes. Chromosoma 101: 553-556.CrossRefGoogle Scholar
  57. Miklos, G.L.G. (1985) Localized highly repetitive sequences in vertebrate and invertebrate genomes. In: Maclntyre R.J. ed. Molecular Evolutionary Genetics. Plenum Press, New York, pp. 241-321.Google Scholar
  58. Miller, T.E., Gerlach, W.L. and Flavell, R.B. (1980) Nucleolus organizer variation in wheat and rye revealed by in situ hybridization. Heredity 45: 377-382.CrossRefGoogle Scholar
  59. Muramoto, N. (1976) A comparative study of C-bands in some species of Heteroptera and Orthoptera. Chrom. Inform. Serv. 20: 34-35.Google Scholar
  60. Orlando, E. (1974) Sex determination in Megoura vicae Bukton (Homoptera, Aphididae). Monit. Zool. 8: 61-70.Google Scholar
  61. Papeschi, A.G. (1988) C-banding and DNA content in three species of Belostoma (Heteroptera) with large differences in chromosome size and number. Genetica 76: 43-51.CrossRefGoogle Scholar
  62. Ray, J.H. and Venketeswaran, S. (1978) Constitutive heterochromatin distribution in monocentric and polycentric chromosomes. Chromosoma 66: 341-350.CrossRefGoogle Scholar
  63. Roussel, P. and Hernandez-Verdum, D. (1994). Identification of Ag-NOR proteins, markers of proliferation related to ribosomal gene activity. Exp. Cell Res. 214: 465-72.PubMedCrossRefGoogle Scholar
  64. Schubert, I. and Wobus, U. (1985) In situ hybridization confirms jumping nucleolus organising regions in Allium. Chromosoma 92: 143-148.CrossRefGoogle Scholar
  65. Schweizer, D. and Loidl, J. (1987) A model for heterochromatin dispersion and the evolution of C-band patterns. Chromosome Today 9: 61-74.Google Scholar
  66. Sen, S. and Khuda-Bukhsh, A.R. (1992) Chromatid separation initiating from the NOR-bearing end of the metaphase chromosomes of two species of Aphis (Homoptera, Aphididae). Cytologia 57: 507-510.Google Scholar
  67. Solari, A.J. (1979) Autosomal synaptonemal complexes and sex chromosomes without axes in Triatoma infestans. Chromosoma 72: 225-240.CrossRefGoogle Scholar
  68. Spence, J.M., Blackman, R.L., Testa, J.M. and Ready, P.D. (1998) A 169-base pair tandem repeat DNA marker for subtelomeric heterochromatin rearrangements in aphids of the Myzus persicae group. Chromosome Res. 6: 167-175.PubMedCrossRefGoogle Scholar
  69. Sumner, A.T., Taggart, M.H., Mezzanotte, R. and Ferrucci, L. (1990) Patterns of digestion of human chromosomes by restriction endonucleases demonstrated by in situ nick translation. Histochem, J. 22: 639-652.CrossRefGoogle Scholar
  70. Tartof, K.D. (1974) Unequal mitotic sister chromatid exchange as the mechanism of ribosomal RNA gene magnification. Proc. Nat. Acad. Sci. USA 71: 1272-1276.PubMedCrossRefGoogle Scholar
  71. Ugarkovic, D., Plhol, M., Petitpierre, E., Lucijanic-Justic, V. and Juan, C. (1994) Tenebrio obscurus satellite DNA is resistant to cleavage by restriction endonuclease in situ. Chromosome Res. 2: 217-223.PubMedCrossRefGoogle Scholar
  72. Vig, B.K. (1987) Sequence of centromere separation. A possible role of repetitive DNA. Mutagenesis 2: 155-159.PubMedCrossRefGoogle Scholar
  73. Wrensch, D.L., Ketheley, J.B. and Norton, R.A. (1994) Cytogenetic of holokinetic chromosomes and inverted meiosis: keys to the evolutionary success of mites with generalization on eukaryotes, In: Houck, M.A. ed. Mites. Ecological and Evolutionary Analysis of Life-History Patterns. Chapman and Hall, New York, London, pp. 282-343.Google Scholar

Copyright information

© Narosa Publishing House, New Delhi, India 2002

Authors and Affiliations

  • Gian Carlo Manicardi
    • 1
  • Mauro Mandrioli
    • 2
  • Davide Bizzaro
    • 3
  • Umberto Bianchi
    • 4
  1. 1.Dipartimento di Biologia AnimaleUniversità di ModenaModenaItaly
  2. 2.Dipartimento di Biologia AnimaleUniversità di ModenaModenaItaly
  3. 3.Dipartimento di Biologia AnimaleUniversità di ModenaModenaItaly
  4. 4.Dipartimento di Biologia AnimaleUniversità di ModenaModenaItaly

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