Abstract
Precipitation of biomolecules such as antibiotics or proteins is a means of concentrating and, in some cases, purifying a desired product. Precipitation of a molecule results in an amorphous solid that is of low purity relative to a product obtained by crystallization. Precipitation is best utilized as a means of product isolation; whereas, crystallization may be an effective polishing step that provides a product of high purity. As in any unit operation, the design engineer must be concerned with equilibrium behavior, kinetic behavior and appropriate equipment selection. In all of these respects, both crystallization and precipitation are more similar than different. However, some differences exist and will be highlighted during the subsequent discussion. Although many of the examples presented in this chapter will focus on the application to protein molecules, the principles applied may be generalized to any system.
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Schall, C.A., Wiencek, J.M. (1997). Product recovery and purification via precipitation and crystallization. In: Goldberg, E. (eds) Handbook of Downstream Processing. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1563-3_8
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DOI: https://doi.org/10.1007/978-94-009-1563-3_8
Publisher Name: Springer, Dordrecht
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