Abstract
Population genetic studies require analysis of many individuals with multiple genetic markers. Prior to the development of the polymerase chain reaction (PCR) insect population geneticists relied almost exclusively on biochemical polymorphisms (Tabachnick and Black, 1996) as genetic markers. Random Amplified Polymorphic DNA (RAPD) and Single-Strand Conformation Polymorphism (SSCP) analysis are two PCR-based techniques developed in the last 5 years that allow a virtually unlimited number of genetic markers to be amplified in individual insects. Both techniques are simple, rapid, safe and relatively inexpensive. They offer the insect geneticist an unprecedented ability to examine many genetic markers simultaneously. This improves resolution of spatial and seasonal genetic differentiation among populations and increases the accuracy and precision of gene flow rate estimates. Monitoring segregation of markers distributed throughout a genome also allows for intensive examination of linkage disequilibrium. RAPD and SSCP markers have already been used in population genetic studies (Puterka et al., 1993; Tabachnick and Black, 1996; Apostol et al., 1996; Norris et al., 1996) and in genetic fingerprinting (Apostol et al., 1993,1994). They have also proven useful in detection and identification of cryptic species (Black et al., 1992; Wilkerson et al., 1993; Kambhampati et al., 1992; Ballinger-Crabtree et al., 1992; Hiss et al., 1994; Black and Munstermann, 1996).
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References
Apostol, B.L., Black IV, W.C., Miller, B.R., Reiter, P. and Beaty, BJ. (1993) Estimation of family numbers at an oviposition site using RAPD-PCR markers: applications to the mosquito Aedes aegypti. Theor. Appl. Genet.86, 991–1000.
Apostol, B.L., Black IV, W.C., Reiter, P. and Miller, B.R. (1994) Use of RAPD-PCR markers to estimate the number of Aedes aegypti families at oviposition sites in San Juan, Puerto Rico. Am. J. Trop. Med. Hyg.51, 89–97.
Apostol, B.L., W.C. Black IV, P. Reiter and Miller, B.R. (1996) Population genetics with RAPD-PCR markers: the breeding structure of Aedes aegypti in Puerto Rico. Heredity76, 325–334.
Ballinger-Crabtree, M.E., Black IV, W.C. and Miller, B.R. (1992) Use of genetic polymorphisms detected by RAPD-PCR for differentiation and identification of Aedes aegypti populations. Am. J. Trop. Med. Hyg.47, 893–901.
Black, W.C. IV (1993) PCR with arbitrary primers: Approach with care. Insect Mol. Biol2, 1–6.
Black, W.C. IV (19%) Statistical analysis of arbitrarily primed PCR patterns in molecular taxo-nomic studies. In Methods in Molecular Biology (CL. Clapp, ed.), pp. 39–55. Humana Press, Totowa, NJ, USA.
Black, W.C. IV and Munstermann, L.M. (19%) Molecular taxonomy and systematics of arthropod vectors. In Biology of Disease Vectors (W.C. Marquardt and B. Beaty, eds). Univ. of Colorado Press. Boulder Co.
Black, W.C, DuTeau, N.M., Puterka, G.J. Nechols, J.R. and Pettorini, J.M. (1992) Use of the Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) to Detect DNA Polymorphisms in Aphids. Bull. Entomol. Res.82, 151–159.
Brower, A.V.Z. and DeSalle, R. (1994) Practical and theoretical considerations for choice of a DNA sequence region in insect molecular systematics, with a short review of published studies using nuclear gene regions. Ann. Entomol. Soc. Am.87, 702–716.
Coen, E.S., Strachan, T. and Dover, G. (1982) Dynamics of concerted evolution of ribosomal DNA and histone gene families in the melanogaster species subgroup of Drosophila. J. Mol. Biol.158, 17–35.
Hayashi, K. (1991) PCR-SSCP: A simple and sensitive method for detection of mutations in genomic DNA. PCR Meth. Appl.1, 34–38.
Hiss, R.H, Noms, D.E., Dietrich, C.H., Whitcomb, R.F., West, D.F., Bosio, C.F., Kambhampati, S., Piesman, J., Antolin M.F. and Black IV, W.C. (1994) Molecular Taxonomy using Single Strand Conformation Polymorphism (SSCP) Analysis of Mitochondrial Ribosomal DNA Genes. Insect Mol Biol.3, 171–182.
Kambhampati, S., Black, W.C. IV and Rai, K.S. (1992) RAPD-PCR for identification and differentiation of mosquito species and populations: Techniques and Statistical analysis. J. Med. Entomol.29, 939–945.
Lynch, M. and Milligan, B.G. (1994) Analysis of population genetic structure with RAPD markers. Mol. Ecol.3, 91–99.
Norris, D.E., Klompen, J.S.H., Keirans, J.E. and Black IV, W.C. (19%) Population genetics of Ixodes scapularis. J. Med. Entomol.33, 78–89.
Orita, M., Iwahana, P.L., Kanazawa, H., Hayashi, K. and Sekiya, T. (1989) Detection of polymorphisms of human DNA by gel electrophoresis as single strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA86, 2766–2770.
Puterka, G. J., Black IV, W.C., Steiner, W.M. and Burton, R.L. (1993) Genetic variation and phylogenetic relationships among worldwide collections of the Russian Wheat Aphid, Diuraphis noxia (Mordvilko), inferred from allozyme and RAPD-PCR markers. Heredity70, 604–618.
Rogers, S.O. and Bendich, A.J. (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol.5, 69–76.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. and Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann. Entomol. Soc. Am.87, 651–701.
Tabachnick, W.J. and Black IV, W.C. (19%) Population genetics of disease vectors. In Biology of Disease Vectors (W.C. Marquardt and B. Beaty, eds), Univ. of Colorado Press, Boulder, CO, USA.
Wilkerson, R.C, Parsons, T.J., Albright, D.G., Klein, T.A. and Braun, M. J. (1993) Random amplified polymorphic DNA (RAPD) markers readily distinguish cryptic mosquito species (Diptera: Culicidae: Anopheles). Insect Mol Biol.1, 205–211.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful genetic markers. Nucl. Acids Res.18, 6531–6535.
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Black, W.C., DuTeau, N.M. (1997). RAPD-PCR and SSCP analysis for insect population genetic studies . In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_31
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DOI: https://doi.org/10.1007/978-94-009-1535-0_31
Publisher Name: Springer, Dordrecht
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