The Molecular Biology of Insect Disease Vectors

A Methods Manual

  • Julian M. Crampton
  • C. Ben Beard
  • Christos Louis

Table of contents

  1. Front Matter
    Pages i-xxv
  2. Care and Maintenance of Insect Colonies

    1. Front Matter
      Pages 1-1
    2. Leonard E. Munstermann
      Pages 13-20
    3. Eddie W. Cupp, Frank B. Ramberg
      Pages 31-40
    4. Ron H. Gooding, Udo Feldmann, Alan S. Robinson
      Pages 41-55
    5. Patricia de Azambuja, Eloi S. Garcia
      Pages 56-64
  3. Experimental Infection of Insect Vectors

    1. Front Matter
      Pages 65-65
    2. Robert E. Sinden
      Pages 67-91
    3. William E. Collins
      Pages 92-100
    4. Harold Townson
      Pages 101-111
    5. Albert E. Bianco, Peter J. Ham
      Pages 121-135
    6. Eloi S. Garcia, Patricia de Azambuja
      Pages 146-155
  4. Basic Methods in Isolating, Cloning and Characterizing Nucleic Acids and their Products

    1. Front Matter
      Pages 157-157
    2. Christos Louis
      Pages 159-163
    3. Margaret A. Hughes
      Pages 164-171
    4. Melanie J. Palmer, William C. Black IV
      Pages 172-194
    5. Margaret A. Hughes
      Pages 195-206
    6. Kostas D. Mathiopoulos
      Pages 207-217
    7. Kostas D. Mathiopoulos
      Pages 218-229
    8. Bruno Arcà, Charalambos Savakis
      Pages 244-260
    9. George Dimopoulos, Christos Louis
      Pages 261-267
    10. Philip W. Ingham, Trevor Jowett
      Pages 268-282
    11. Marcos A. González-Gaitán, Herbert Jäckle
      Pages 283-294
    12. Hans-Michael Müller
      Pages 295-306
  5. Genome Mapping Techniques

    1. Front Matter
      Pages 307-307
    2. David W. Severson
      Pages 309-320
    3. Liangbiao Zheng
      Pages 321-328
    4. Alessandra della Torre
      Pages 329-336
    5. Vasantha Kumar, Anthony J. Cornel, Odette Mukabayire
      Pages 337-345
  6. Insect Identification Techniques

    1. Front Matter
      Pages 359-359
    2. William C. Black IV, Nancy M. DuTeau
      Pages 361-373
    3. Susan M. Paskewitz, Frank H. Collins
      Pages 374-383
    4. Nicole Pasteur, Michel Raymond, François Rousset, Jean-Baptiste Bergé, Marcel Amichot, David Pauron et al.
      Pages 399-419
  7. Transformation Techniques and Viral Systems

    1. Front Matter
      Pages 421-421
    2. Alison C. Morris
      Pages 423-429
    3. Ann Marie Fallon
      Pages 430-443
    4. Jon O. Carlson, Boris N. Afansiev, Stephen Higgs, Tomoyo Matsubara, Jane C. Burns
      Pages 444-458
    5. Stephen Higgs, Ken E. Olson, Kurt I. Kamrud, Ann M. Powers, Barry J. Beaty
      Pages 459-483
  8. Cell and Organ Culture

    1. Front Matter
      Pages 485-485
    2. Alison C. Morris, Anthony A. James
      Pages 500-506
    3. Alexander S. Raikhel, Kirk W. Deitsch, Thomas W. Sappington
      Pages 507-522
    4. Thomas W. Sappington, Mark R. Brown, Alexander S. Raikhel
      Pages 523-535

About this book


Only one generation ago, entomology was a proudly isolated discipline. In Comstock Hall, the building of the Department of Entomology at Cornell University where I was first introduced to experimental science in the laboratory of Tom Eisner, those of us interested in the chemistry of life felt like interlopers. In the 35 years that have elapsed since then, all of biology has changed, and entomology with it. Arrogant molecular biologists and resentful classical biologists might think that what has happened is a hostile take-over of biology by molecular biology. But they are wrong. More and more we now understand that the events were happier and much more exciting, amounting to a new synthesis. Molecular Biology, which was initially focused on the simplest of organisms, bacteria and viruses, broke out of its confines after the initial fundamental questions were answered - the structure of DNA, the genetic code, the nature of regulatory genes - and, importantly, as its methods became more and more generally applicable. The recombinant DNA revo­ lution of the 1970s, the development of techniques for sequencing macromolecules, the polymerase chain reaction, new molecular methods of genetic analysis, all brought molecular biology face to face with the infinite complexity and the exuber­ ant diversity of life. Molecular biology itself stopped being an isolated diScipline, pre­ occupied with the universal laws of life, and became an approach to addressing fas­ cinating specific problems from every field of biology.


biology development genome mapping molecular biology molecular techniques parasites

Editors and affiliations

  • Julian M. Crampton
    • 1
  • C. Ben Beard
    • 2
  • Christos Louis
    • 3
  1. 1.Division of Molecular Biology and Immunology, Wolfson Unit of Molecular GeneticsLiverpool School of Tropical MedicineLiverpoolUK
  2. 2.Division of Parasitic DiseasesCenters for Disease Control and PreventionAtlantaUSA
  3. 3.Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Hellas and Department of BiologyUniversity of CreteHeraklionGreece

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 1997
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-94-010-7185-7
  • Online ISBN 978-94-009-1535-0
  • Buy this book on publisher's site
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