Abstract
Techniques for the localization of mRNAs in situ were first developed in the late 1970s and are now widely used in many areas of biological research. They provide an extremely powerful tool for analysing patterns of gene expression, both temporally and spatially. Although other very sensitive methods are available for assaying transcript levels in different tissues and at different developmental or physiological stages (Chapters 19, 20 and 22), in situ hybridization is unique in its ability to reveal patterns of gene expression with cellular resolution.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Akam, M.E. (1983) The localisation of Ultrabithorax transcripts in Drosophila tissue sections. EMBO J.2, 2075–2084.
Curtis, D. Apfeld, J. and Lehman, R. (1995) nanos is an evolutionarily conserved organiser of anterior-posterior polarity. Development121, 1899–1910.
Hafen, E., Levine, M., Garber, R.L. and Gehring, W.J. (1983) An improved in situ hybridisation method for the detection of cellular RNAs in Drosophila tissue sections and its application for localising transcripts of the homeotic Antennapedia gene complex. EMBO J.2, 617–623.
Ingham, P.W. (1988) The molecular genetics of embryonic pattern formation in Drosophila. Nature335, 25–33.
Ingham, P.W., Howard, K.R. and Ish-Horowicz, D. (1985) Transcription patterns of the Drosophila segmentation gene hairy. Nature318, 439–445.
Ingham, P.W., Hidalgo, A. and Taylor, A.M. (1990) Advantages and limitations of in situ hybridisation as exemplified by the molecular genetic analysis of Drosophila development. In In situ hybridisation: application to developmental biology and medicine (N. Harris and D.G. Wilkinson, eds), pp. 97–114. Cambridge University Press, Cambridge.
Sommer, R. and Tautz, D. (1991) Segmentation gene expression in the housefly, Musca domestica. Development113, 419–430.
Tautz, D. and Pfeifle, C. (1989) A non-radioactive in situ hybridisation method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma98, 81–85.
Tautz, D., Hulskamp, M. and Sommer, R.J. (1992) Whole mount in situ hybridisation in Drosophila. In In situ hybridisation: A Practical Approach (D.G. Wilkinson, ed.), pp. 61–73. IRL Press, Oxford.
Wilkinson, D.G. (1992) The Theory and Practice of In situ Hybridisation. In In situ hybridisation: A Practical Approach (D.G. Wilkinson, ed.), pp 1–13. IRL Press, Oxford.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Chapman & Hall
About this chapter
Cite this chapter
Ingham, P.W., Jowett, T. (1997). Localization of RNA transcripts by in situ hybridization. In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_23
Download citation
DOI: https://doi.org/10.1007/978-94-009-1535-0_23
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-7185-7
Online ISBN: 978-94-009-1535-0
eBook Packages: Springer Book Archive