Abstract
Most biological processes rely on changes in the level and pattern of gene expression. Their molecular and functional dissection often requires the isolation and subsequent characterization of genes that are differentially expressed in space (different tissues or organs), in time (different developmental stages), or in only one sex. Only 15% of the genes are expressed in all cells, and even fewer are constitutively expressed exhibiting no temporal specificity at all. Until recently, there were only two alternative approaches for the isolation of differentially expressed genes: differential hybridization using various probes, or the use of subtractive cDNA libraries. Both methods are time-consuming and require large amounts of starting material, especially for the construction of the libraries.
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References
Bauer, D., Muller, H., Reich, J., Riedel, H., Ahrenkiel, V., Warthoe, P. and Strauss, M. (1993) Identification of differentially expressed mRNA species by an improved display technique. Nucl. Acids Res.18, 4272–4280.
Li, F., Barnathan, E.S. and Kariko, K. (1994) Rapid method for screening and cloning cDNAs generated in differential mRNA display: application of Northern blot for capturing of cDNAs. Nucl Acids Res.22, 1764–1765.
Liang, P. and Pardee, A.B. (1992) Differential display of eukaryotic messenger RNA by means of the Polymerase Chain Reaction. Science257, 967–971
Marchuk, D., Drumm, M., Saulino, A. and Collins, F.S. (1991) T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucl Acids Res.19, 1154.
Noriega, F.G. and Wells, A. (1993) A comparison of three methods for isolating RNA from mosquitoes. Insect Mol Biol.2, 21–24.
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Sokolov, B.P. and Prockop, D.J. (1994) A rapid and simple PCR-based method for isolation of cDNA from differentially expressed genes. Nucl Acids Res.22, 4009–4015.
Welsh, J., Chada, K., Dalal, S.S., Cheng, R., Ralph, D. and McClelland, M. (1992) Arbitrary primed PCR fingerprinting of RNA. Nucl Acids Res.19, 4965–4970.
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Dimopoulos, G., Louis, C. (1997). Differential display of mRNA . In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_22
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DOI: https://doi.org/10.1007/978-94-009-1535-0_22
Publisher Name: Springer, Dordrecht
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