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A Stable Enzyme Biosensor for Determination of Glucose

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Biotechnology Research and Applications

Abstract

Many methods for determination of glucose have limitations with respect to the stability, reproducibility and speed of response, as well as the range of glucose concentration accessible. A new glucose oxidase electrode is described which suffers from none of these disadvantages, and promises greater scope for amperometric glucose determinations in various biomedical and industrial applications. The electrode is easily prepared by immobilisation of the enzyme on platinised carbon, and requires no mediator. Transfer of electrons from the enzyme to the electrode, poised at a fixed potential, is catalysed rapidly and efficiently by a mechanism which involves adsorbed oxygen, but the response is effectively independent of oxygen tension in the sample.

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© 1988 Elsevier Applied Science Publisher Ltd

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Bennetto, H.P. et al. (1988). A Stable Enzyme Biosensor for Determination of Glucose. In: Gavora, J., Gerson, D.F., Luong, J., Storer, A., Woodley, J.H. (eds) Biotechnology Research and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1371-4_18

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  • DOI: https://doi.org/10.1007/978-94-009-1371-4_18

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7111-6

  • Online ISBN: 978-94-009-1371-4

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