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Adsorption-Desorption of Proteins in RP-HPLC RNase on C4 Supports

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Separations for Biotechnology 2

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Abstract

The adsorption-desorption of RNase on a C4 RP-HPLC support has been investigated in order to elucidate the effect of the solvent (isopropyl alcohol) and of the ion pairing (trifluoroacetic acid) on the equilibrium data. A fundamental role of the ion pairing has been evidenced by the experimental data. The adsorption equilibrium data have been described using a multicomponent Langmuir-Freundlich type isotherm.

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Author information

Authors and Affiliations

  1. Eniricerche S.p.A., Via E. Ramarini, 32-00015, Monterotondo (Roma), Italy

    Ezio D’ Addario & Michele Patrissi

  2. Dipartimento di Chimica Fisica Applicata, Politecnico di Milano, P.za Leonardo da Vinci, 32-20133, Milano, Italy

    Maurizio Masi, Giuseppe Storti & Sergio Carra’

Authors
  1. Ezio D’ Addario
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  2. Michele Patrissi
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  3. Maurizio Masi
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  4. Giuseppe Storti
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  5. Sergio Carra’
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Editor information

Editors and Affiliations

  1. Biotechnology Group, University of Reading, UK

    D. L. Pyle

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© 1990 SCI

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D’ Addario, E., Patrissi, M., Masi, M., Storti, G., Carra’, S. (1990). Adsorption-Desorption of Proteins in RP-HPLC RNase on C4 Supports. In: Pyle, D.L. (eds) Separations for Biotechnology 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0783-6_33

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  • DOI: https://doi.org/10.1007/978-94-009-0783-6_33

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6839-0

  • Online ISBN: 978-94-009-0783-6

  • eBook Packages: Springer Book Archive

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