Skip to main content

Solid Phases for Immunoaffinity Adsorption

Physical and biochemical influences upon preparative and analytical performance

  • Chapter
Separations for Biotechnology 2

Abstract

The application of novel silica and polymer supports in the construction of immunoaffinity adsorbents is described. The influence of surface chemistry, particle geometry, ligand and product size, and stoichiometry of adsorptive association is considered in respect of preparative and analytical applications of immunoaffinity adsorption. Practical requirements for an improved understanding of physical and chemical constraints upon the optimal design of efficient and productive bioselective adsorbents are discussed in the context of preparative and analytical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Katoh, S., Scaling-up affinity chromatography, TIBTECH, 1987, 5, 281–286.

    Google Scholar 

  2. .Hill, E.A. and Hirtenstein, M.D., Affinity Chromatography: its application to industrial scale processes. In Advances in Biotechnological Processes, Alan Liss, N.Y., 1983, 1, 31–66.

    Google Scholar 

  3. .Clonis, Y.D., Large-scale affinity chromatography, Bio/Technology, 5, 1290–1293.

    Google Scholar 

  4. Lyddiatt, A., Solid phases and product contactors: new options for bioselective adsorption. In World Biotechnology Report, Online Publications. London, UK, 1988, 167–172.

    Google Scholar 

  5. .Groman, E.V. and Wilchek, M., Recent developments in affinity chromatography supports. TIBTECH, 1987, 5, 220–224.

    CAS  Google Scholar 

  6. .Kennedy, J.F., Rivera,, Z.S. and White, C.A., The use of HPLC in biotechnology. J. Biotechnology, 1989, 9, 83–106.

    Article  CAS  Google Scholar 

  7. .Porschka, M., Universal calibration of gel permeation chromatography and determination of molecular shape in solution. Anal. Biochem., 1987, 162, 42–64.

    Google Scholar 

  8. .Attwood, T.K., Nelmes, B.J. and Sellen, D.B, Electron micrsocopy of beaded agarose gels, 1988. Biopolymers, 27, 201–212.

    Article  CAS  Google Scholar 

  9. .Rudge, J., Desai, M.A., Shojaosadaty, S.A. and Lyddiatt, A., Continuous culture of murine hybridomas with integrated recovery of monoclonal antibodies. In Modern Approaches to Animal Cell Technology, ed, R.E. Spier and J.B. Griffiths, Butterworth, London, 1987, pp.556–575.

    Google Scholar 

  10. .Desai, M.A., Huddleston, J.G., Lyddiatt, A., Rudge, J. and Stevens, A.B., Biochemical and physical characterisation of a composite solid phase. In Separations for Biotechnology, ed M.S. Verrall and M.J. Hudson, Ellis-Horwood, Chichester, UK, 1987, pp.200–209.

    Google Scholar 

  11. .Lyddiatt, A., Desai, M.A., Huddleston, J.G., Rudge, J and Shojaosadaty, S.A., Controlled assembly and operation of immunoadsorbents. J. Chem. Tech. Biotech., (1989), 45, 47–60.

    Google Scholar 

  12. Desai, M.A. and Lyddiatt, A., Comparative studies of agarose and kieselguhr-agarose composites for the preparation and operation of immunosorbents. Bioseparation, 1990, In press.

    Google Scholar 

  13. .Chappell, I., Preparative media design. Laboratory Practice, 1988, 36, 61–64.

    Google Scholar 

  14. .Bethell, G.S., Ayers, J.S., Hancock, W.S. and Hearn, M.T.W., A novel method of activation of cross-linked agaroses which gives a matrix for affinit chrommatography devoid of additional charged groups. J. Biol. Chem., 1979, 254, 2572–2574.

    PubMed  CAS  Google Scholar 

  15. Chase, H. A., Prediction of the performance of preparative affinity chromatography. J Chromatog., 297, 179-202.

    Google Scholar 

  16. .Goding, J.W., Monoclonal Antobodies: Principles and Practice, Academic Press., London,. 1983, pps 98–127.

    Google Scholar 

  17. .Voller, A., Bidwell, D.E. and Bartlett, A., Enzymatic immunoassays in diagnostic medicine: theory and practice. Bulletin of World Health Organisation, 1976, 53, 55–65.

    CAS  Google Scholar 

  18. .Pharmacia Technical Literature, Uppsala, Sweden, 1989.

    Google Scholar 

  19. .Larsson, P-O., High performance affinity chromatography. In Methods in Enzymology, 1989, 18 209–220.

    Google Scholar 

  20. .Burg, B.V.D., Eijsink, V.G.M., Stulp, B.K. and Venemam, G., One step affinity purification of Bacillus neutral proteases using Bacitracinsilica. J. Biochem. Biophys. Methods, 1989, 18, 209–220.

    Article  Google Scholar 

  21. .Shojaosadaty, S.A. and Lyddiatt, A. Application of affinity HPLC to recovery and monitoring operations in biotechnology, In Separations for Biotechnology, ed. M.S. Verrall and M.J. Hudson, Ellis-Horwood, Chichester, UK, 1987, pp 436–444.

    Google Scholar 

  22. Malhotra, J.M., Characterisation and application of a novel solid phase for bioaffinity HPLC. M. Phil. Thesis, University of Birmingham, 1989.

    Google Scholar 

  23. Fowell, S.L. and Chase, H.A., A comparison of some activated matrices for preparation of immunoadsorbents. J. Biotechnol., 1986, 355-368.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1990 SCI

About this chapter

Cite this chapter

Mohan, S.B., Malhotra, J.M., Lyddiatt, A. (1990). Solid Phases for Immunoaffinity Adsorption. In: Pyle, D.L. (eds) Separations for Biotechnology 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0783-6_22

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-0783-6_22

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6839-0

  • Online ISBN: 978-94-009-0783-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics