Abstract
The assembly and characterisation of beaded forms of agar (100-500 μm) formed in simple, economic emulsification procedures is described. Such particles have been chemically cross-linked to enhance physical, temperature and pH stabilities, and have been subsequently derivatised with ion-exchange groups, textile dyes and blood antigens to form selective adsorbents. Materials have been characterised in single-step, fixed bed concentrations and/or purifications of albumin from solution and monoclonal antibodies from animal cell culture broths. The introduction of inert, dense particles during bead emulsification has yielded material suited to similar product recoveries from biological suspensions in fluidised bed contactors. Direct comparison has been made with conventional commercial chromatographic materials in terms of performance efficiency and cost effectiveness in realistic biorecoveries.
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Gibson, N.B., Lyddiatt, A. (1990). Economic Fabrication and Utilisation of Agar Composites in Bioselective Recovery in Fixed and Fluidised Beds. In: Pyle, D.L. (eds) Separations for Biotechnology 2. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0783-6_17
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DOI: https://doi.org/10.1007/978-94-009-0783-6_17
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