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Recovery and Purification of Antibody

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Antibody Expression and Production

Part of the book series: Cell Engineering ((CEEN,volume 7))

Abstract

Monoclonal antibody drugs have become a large portion of protein therapeutics and many antibody molecules are being evaluated at various stages of clinical trials in the biopharmaceutical industry. This review article summarizes the state of the art antibody purification techniques. The main focus is chromatographic techniques that include protein A, ion exchange and HIC. For each technique, the mechanisms of antibody binding, factors affecting binding capacity, resin selection, as well as typical process parameters and chromatograms are discussed in detail. Cell culture clarification, filtration, alternative antibody purification techniques, and viral clearance strategies are discussed briefly. The goal of this article is to provide a broad coverage of antibody purification technology. Readers are referred to extensive articles for further in-depth reading.

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Acknowledgements

The authors are grateful to Yinges Yigzaw at Amgen for his feedbacks during the preparation of the chapter. We also would like to thank Priyanka Gupta at Sartorius Stedim North America Inc. for the permission to use Fig. 14.9.

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  1. Amgen Inc., Seattle, WA, 98119, USA

    XueJun Han, Arthur Hewig & Ganesh Vedantham

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Correspondence to Ganesh Vedantham .

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  1. Dept. Chemical & Biochemical, Engineering, University College, Dublin, Belfield, Dublin 4, Ireland

    Mohamed Al-Rubeai

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Han, X., Hewig, A., Vedantham, G. (2011). Recovery and Purification of Antibody. In: Al-Rubeai, M. (eds) Antibody Expression and Production. Cell Engineering, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1257-7_14

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